• Specific Circular Junction Probes
Reliably and accurately identify individual circRNAs, even in the presence of their linear counterparts (Fig. 1).
Figure 1. Arraystar circRNA Array V2.0 uses specific circular junction probes to accurately and reliably detect each individual circRNAs, even in the presence of their linear counterparts. The linear RNA at the bottom is alternatively processed to generate a circular variant above. A probe is designed to target the circRNA-specific junction site, where the 5' end of exon A joins together with the 3' end of exon B.
• Detailed annotation for circRNA-miRNA association
Annotation of potential miRNA target sites on the circular RNAs helps to unravel their functional roles as a natural miRNA sponge (Fig. 2).
Figure 2. The association between circular RNA and conserved miRNAs is annotated in detail.
• The preferred choice over RNA-sequencing, as RNA-seq is currently inadequate for such task due to the particular properties of circular RNA. Learn more >
1) Circular junction read counts are only a fraction of the circular RNA. They are much lower than the linear RNA at the same abundance level.
2) For detection of the circular RNA presence, a few reproducible counts are required. But for quantification, at least hundreds of read counts are required.
3) Even with combined large datasets and studies, most of the known circular RNAs have just a few read counts. Count numbers at these levels are not nearly sufficient for differential expression analysis.
In short, circular RNA sequencing can be used for novel discovery by a few read counts, but is inadequate for differential expression analysis even at modest abundance levels.
Figure 3. RNA-seq quantification reliability vs read depth. Typical RNA-seq has a depth of < 30 mil reads for mRNAs (blue circle), which is < 0.5 mil for cross circular junction reads (red circle). Less than 5% circular junctions can be reliably quantified. Adopted from Labaj et al, (2011) Bioinformatics [PMID 21685096].
• Guaranteed performance
- Better sensitivity: Low abundance RNAs are accurately detected with a wide dynamic range of over 5 orders of magnitude (Fig. 4).
Figure 4. A wide dynamic detection range of over 5 orders of magnitude with Arraystar's circRNA Arrays.
- High Reproducibility: Technical replicates show tight correlation on Arraystar circRNA Arrays (R2>0.9) (Fig. 5).
Figure 5. High reproducibility on Arraystar circRNA arrays.
TGF-ß signaling promotes cervical cancer metastasis via CDR1as. Zhong G, et al. Molecular Cancer, 2023
CircRNA DICAR as a novel endogenous regulator for diabetic cardiomyopathy and diabetic pyroptosis of cardiomyocytes. Yuan Q, et al. Signal Transduction and Targeted Therapy, 2023
Exosomal circTUBGCP4 promotes vascular endothelial cell tipping and colorectal cancer metastasis by activating Akt signaling pathway. Chen C, et al. Journal of Experimental & Clinical Cancer Research, 2023
Cytoskeleton remodeling mediated by circRNA-YBX1 phase separation suppresses the metastasis of liver cancer. Liu B, et al. Proceedings of the National Academy of Sciences?, 2023
CircOGDH Is a Penumbra Biomarker and Therapeutic Target in Acute Ischemic Stroke. Liu Y, et al. Circulation Research, 2022
CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. Liu L, et al. Molecular Cancer, 2022
The N6-methyladenosine modification of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway. Lin C, et al. Molecular Cancer, 2022
Hsa_circ_0003258 promotes prostate cancer metastasis by complexing with IGF2BP3 and sponging miR-653-5p. Yu Y Z, et al. Molecular Cancer, 2022
circCAPRIN1 interacts with STAT2 to promote tumor progression and lipid synthesis via upregulating ACC1 expression in colorectal cancer. Yang Y, et al. Cancer Communications, 2022
The circular RNA circDLG1 promotes gastric cancer progression and anti-PD-1 resistance through the regulation of CXCL12 by sponging miR-141-3p. Chen D L, et al. Molecular Cancer, 2021
Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Gu Y, et al. Molecular Cancer, 2021
CircRNF220, not its linear cognate gene RNF220, regulates cell growth and is associated with relapse in pediatric acute myeloid leukemia. Liu X, et al. Molecular Cancer, 2021
The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC. Wang X, et al. Molecular Cancer, 2021
CircMEMO1 modulates the promoter methylation and expression of TCF21 to regulate hepatocellular carcinoma progression and sor*****b treatment sensitivity. Dong Z R, et al. Molecular Cancer, 2021
Circular RNA ACTN4 promotes intrahepatic cholangiocarcinoma progression by recruiting YBX1 to initiate FZD7 transcription. Chen Q, et al. Journal of Hepatology, 2021
Circular RNA circHIPK3 promotes homeostasis of the intestinal epithelium by reducing miR-29b function. Xiao L, et al. Gastroenterology, 2021
Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output. Qiyi Zhao, et al. Cell, 2020
Extracellular Vesicle-Mediated Delivery of CircSCMH1 Promotes Functional Recovery in Rodent and Nonhuman Primate Ischemic Stroke Models. Yang L, et al. Circulation, 2020
1972P Circular RNA is associated with enz***amide resistant prostate cancer. Lim M C J, et al. Annals of Oncology, 2020
Circular RNAs as biomarkers in liquid biopsy in colorectal cancer. Valladares-Ayerbes M, et al. Journal of Clinical Oncology, 2020
A psychiatric disease-related circular RNA controls synaptic gene expression and cognition. Zimmerman A J, et al. Molecular Psychiatry, 2020
CircPTK2 (hsa_circ_0005273) as a novel therapeutic target for metastatic colorectal cancer. Yang H, et al. Molecular Cancer, 2020
Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals. Xue D, et al. Molecular Cancer, 2019
circGSK3ß promotes metastasis in esophageal squamous cell carcinoma by augmenting ß-catenin signaling. Hu X, et al. Molecular Cancer, 2019
A Noncoding Regulatory RNAs Network Driven by Circ-CDYL Acts Specifically in the Early Stages Hepatocellular Carcinoma. Wei Y, et al. Hepatology, 2019
Circular RNA CircFndc3b modulates cardiac repair after myocardial infarction via FUS/VEGF-A axis. Garikipati V., et al. Nature Communications, 2019
FOXP1 circular RNA sustains mesenchymal stem cell identity via microRNA inhibition. Cherubini A, et al. Nucleic Acids Research, 2019
Circular RNA cESRP1 sensitises small cell lung cancer cells to chemotherapy by sponging miR-93-5p to inhibit TGF-ß signalling. Huang W, et al. Cell Death & Differentiation, 2019
A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Xia P, et al. Immunity, 2018
PRMT5 circular RNA promotes metastasis of urothelial carcinoma of the bladder through sponging miR-30c to induce epithelial-mesenchymal transition. Chen X, et al. Clinical Cancer Research, 2018
circEPSTI1 as a prognostic marker and mediator of triple-negative breast cancer progression. Chen B, et al. Theranostics, 2018
Circular RNA MTO1 acts as the sponge of miR-9 to suppress hepatocellular carcinoma progression. Han D, et al. Hepatology (Baltimore, Md.), 2017
A Circular RNA Binds To and Activates AKT Phosphorylation and Nuclear Localization Reducing Apoptosis and Enhancing Cardiac Repair. Zeng Y, et al. Theranostics, 2017
circRNA Mediates Silica-Induced Macrophage Activation Via HECTD1/ZC3H12A-Dependent Ubiquitination. Zhou Z, et al. Theranostics, 2017
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