Arraystar Small RNA Modification Array Service quantifies your choice of 8-oxoguanine (o8G), 7-methylguanosine (m7G), N6-methyladenosine (m6A), Pseudouridine (Ψ), or 5-methylcytidine (m5C) modification in miRNAs, pre-miRNAs, and tRNA-derived small RNAs (tsRNAs, including tRFs and tiRNAs) on a single array. Please specify one modification per microarray experiment when you request a quote.

Benefits

•  Coverage of multiple small RNA classes, including miRNAs, pre-miRNAs, and tsRNAs (tRFs and tiRNAs)

•  Gold standard for accurate quantification of modified small RNAs
Direct RNA end labeling ensures high fidelity of quantification, without the problem of modification blocked cDNA synthesis during RNA-seq library prep.

•  High sensitivity for modified small RNAs at lower levels
Overcome the limitations of sequencing technology, excellent analytical sensitivity for small RNAs at low levels of expression or modification.  

•  Low sample amount required, starting from as little as 1 µg total RNA.

Watch Video> How to Profile Small RNA Modifications?
 

Human Small RNA Modification Array V1.0

Mouse Small RNA Modification Array V1.0

References

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The data analysis includes ready-to-use key measurements, useful annotations and publication quality graphics.

Differentially Modified miRNAs, pre-miRNAs, and tsRNAs (tRFs and tiRNAs)

MatureID:  miRBase ID for the mature miRNA.

Group m7G miRNA level (normalized, log2):  Group averaged, log2 transformed, normalized m7G modified miRNA level, based on the Cy5 signal intensities of the m7G-IP RNA in the sample group.

Treated, Control:  The “treated” and “Control” sample groups.

FC:  The fold change comparing the sample groups.

P:  p-value by t-test, for the statistical significance of the difference.

Regulation: Up- or down-regulation by comparing the sample groups.

Group m7G %modified miRNA: Group averaged percentage of m7G modified miRNA for the sample group. miRNA_family: miRNA family members having the same seed sequence.

m7G_motif: “RAm7GGT” motif sequence of the m7G site, where R represents G or A.

Hierarchical clustering heatmap of differentially modified miRNAs, pre-miRNAs, and tsRNAs (tRFs and tiRNAs)

Figure 1. Hierarchical clustering heatmap of differentially modified small RNAs. The modified RNA levels are represented by red-blue color scale referenced in the color key on the top left. The top dendrogram shows the relative closeness of the modification profiles among the samples. The sample group membership is indicated by the color bars above the heat map.