piRNA Array Service

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Arraystar piRNA Arrays are designed to profile tens of thousands of piRNAs at high specificity and sensitivity, for the study of piRNA biology, molecular functions, and biomarker applications. We offer the complete piRNA array service, from sample to publishable data!


• Comprehensive collection and reliable selection of piRNA sequences.

• 60-mer oligonucleotide probes using duplex design to achieve high specificity.

• Duplicated probes for most of the piRNAs.

Service NameCatalog NoDescriptionFormatPrice
Arraystar Mouse piRNA Array AS-S-piR-M Detected 43,537 piRNAs 4*44K
Arraystar Human piRNA Array AS--S-piR-H Detected 23,677 piRNAs 4*44K
Arraystar Rat piRNA Array AS-S-piR-R Detected 39,727 piRNAs 4*44K

Piwi-interacting RNAs (piRNAs) are the largest class of single stranded, small non-coding RNAs of about 26-32 nucleotides in length. piRNAs interact with the Piwi (P-element Induced Wimpy Testis) subfamily of Argonaute proteins. The Piwi subfamily comprises Piwi, Aubergine and AGO3 in flies, MILI, MIWI and MIWI2 in mice, and HILI, HIWI1, HIWI2 and HIWI3 in humans. Individual piRNAs are very diverse and are poorly conserved even between closely related species. In contrast to several hundred microRNA species, tens of thousands of unique piRNA sequences are known in human, mouse and rat. piRNAs are strikingly different from microRNAs in their length, expression pattern, genomic organization and biogenesis.

Arraystar's scientists have developed piRNA arrays for human, mouse and rat species to evaluate the global expression of piRNAs. The piRNA sequences from all three organisms are downloaded from the NCBI database, and mapped to the genome sequences (HG19, MM9 and RN4) using UCSC Blat. Antisense 60-mer oligonucleotide probes are designed using a duplex method. Our piRNA arrays sensitively and accurately profile the expression of piRNAs.

Microarray Arraystar Human piRNA Array Arraystar Mouse piRNA Array Arraystar Rat piRNA Array
Species human mouse rat
Reference genome HG19 MM9 RN4
Number of piRNAs 23,677 43,537 39,727
Probe length 60 nt 60 nt 60 nt
Labeling method Ligation of Cy3 label with dephosphorylated RNA by T4 RNA ligase
Array format 4 * 44K 4 * 44K 4 * 44K

Arraystar scientists have extensive experience with microarray data processing and analysis. piRNA array raw data points are extracted, QC flagged, and quantile normalized. The gene expression values and annotations are compiled. Differentially expressed piRNAs are identified by the fold change and statistical significance.
Figure. piRNA array data analyses and graphic display items are included in the standard analysis package.

Please refer to Sample Submission for details on how to get your project started.

• RNA isolation (Optional)


• Target reparation by labeling with Cy3

• Array hybridization, washing, and scanning

• Data extraction, analysis and summarization

PIWI-interacting RNA-YBX1 inhibits proliferation and metastasis by the MAPK signaling pathway via YBX1 in triple-negative breast cancer. Wu L, et al. Cell Death Discovery, 2024

Cadmium disrupts spermatogenic cell cycle via piRNA-DQ717867/ p53 pathway. Wei J, et al. Reproductive Toxicology, 2024

piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination. Zhang W, et al. Experimental & Molecular Medicine, 2023

P-Element-Induced Wimpy Testis Proteins and P-Element-Induced Wimpy Testis-Interacting RNAs Expression in Ovarian Cancer Stem Cells. Sohn E J, et al. Genetic Testing and Molecular Biomarkers, 2023

piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation. Subhramanyam C S, et al. Molecular Neurobiology, 2022

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N 6-methyladenosine methylation of Parp10 mRNA. Gao X Q, et al. Nature Cell Biology, 2020

PIWIL3/OIP5-AS1/miR-367-3p/CEBPA feedback loop regulates the biological behavior of glioma cells. Liu X,  et al. Theranostics, 2018

RASSF1C modulation of Piwi-interacting RNAs (piRNAs) in lung cancer.YG Amaar, et al.Cancer Research, 2015

Roles of piRNAs in microcystin-leucine-arginine (MC-LR) induced reproductive toxicity in testis on male offspring. Zhang L, et al. Food and Chemical Toxicology, 2017    

Piwi-interacting RNAs as novel prognostic markers in clear cell renal cell carcinomas. J Busch, et al. J Exp Clin Cancer Res, 2015

Altered Expression of PIWI RNA in the Rat Brain After Transient Focal Ischemia. Ashutosh Dharap, Venkata Prasuja Nakka and Raghu Vemuganti. STROKEAHA, 2010.

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