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NuRNA™ Human Central Metabolism PCR Array AS-NM-004-1 384-well plate $375.00
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Metabolism is the foundation of living cells and impacts all cellular functions. Metabolic pathway architecture varies profoundly with cellular conditions, such as cell growth, proliferation, differentiation and death [1, 2], which is mainly shaped up by the cell type functions and environment. Diverse mechanisms including signaling pathways regulate metabolism, and reciprocally, altered metabolism can act as signals and retool the cells independently [3]. Recent discoveries have shed new light on the metabolic control of multitude of cellular processes such as epigenetics [4, 5], autophagy [6, 7], apoptosis and regulated necrosis [8]. Abnormal metabolism is the underlying cause of some of the most prevalent diseases such as obesity, diabetes and cancer (Figure 1). At cellular level, proliferating or cancer cells characteristically rewire signaling and reprogram metabolic pathways to fulfill continuous anabolic high demands (Figure 2). Systematic understanding of metabolic changes in these diseases will greatly advance the science behind, identify metabolic biomarkers and devise new therapeutic targets.

Figure_1_schematic_of_cell_metabolism

Figure 1. Metabolic impacts of cellular processes and association with diseases.

Arraystar NuRNA™ Human Central Metabolism PCR Array is specifically designed for rapid and convenient expression profiling of 373 transcripts for the enzymes and proteins in the core metabolic pathways and crucial metabolites transport systems. It is a systematic and powerful tool for hypothesis-driven and exploratory analysis in metabolism research. The array covers the core metabolic pathways and metabolite transport systems include glucose transporters, glycolysis, TCA cycle, glycerol/fatty acid/cholesterol synthesis, lactate production and transporters, gluconeogenesis, glycogen metabolism, hexosamine metabolism, pentose phosphate pathway, serine/glycine/one-carbon metabolism, glutamine transporters and glutaminolysis, redox balance/GSH synthesis, fatty acid oxidation, acetate metabolism, and nucleotide metabolism. Each assay is rigorously validated across numerous tissues and cell lines. To ensure the upmost data quality, the array includes Spike-in control, Positive PCR Control, Genomic DNA Control and normalization references. 

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Figure 2. Schematic review of cancer metabolism.

 

References

1. Agathocleous, M. and Harris, W. A. (2013) Metabolism in physiological cell proliferation and differentiation. Trends Cell Biol, 23(10):484-92 [PMID: 23756093]
2. Green, D. R., et al. (2014) Cell biology. Metabolic control of cell death. Science, 345(6203):1250256 [PMID: 25237106]
3. Metallo, C. M. and Vander Heiden, M. G. (2013) Understanding metabolic regulation and its influence on cell physiology. Mol Cell, 49(3):388-98 [PMID: 23395269]
4. Lu, C. and Thompson, C. B. (2012) Metabolic regulation of epigenetics. Cell Metab, 16(1):9-17 [PMID: 22768835]
5. Kinnaird, A., et al. (2016) Metabolic control of epigenetics in cancer. Nat Rev Cancer, 16(11):694-707 [PMID: 27634449]
6. Galluzzi, L., et al. (2014) Metabolic control of autophagy. Cell, 159(6):1263-76 [PMID: 25480292]
7. Kaur, J. and Debnath, J. (2015) Autophagy at the crossroads of catabolism and anabolism. Nat Rev Mol Cell Biol, 16(8):461-72 [PMID: 26177004]
8. Vanden Berghe, T., et al. (2014) Regulated necrosis: the expanding network of non-apoptotic cell death pathways. Nat Rev Mol Cell Biol, 15(2):135-47 [PMID: 24452471]

Human metabolic enzymes and metabolite transporters (373)

Glucose transporters:

SLC2A1, SLC2A2, SLC2A3, SLC2A4, SLC2A5, SLC2A6, SLC2A7, SLC2A8, SLC2A9, SLC2A10, SLC2A11, SLC2A12, SLC2A14

Glycolysis:

ADPGK, ALDOA, ALDOB, ALDOC, BPGM, ENO1, ENO2, ENO3, GAPDH, GAPDHS, GCK, GPI, HK1, HK2, HK3, HKDC1, PFKFB1, PFKFB2, PFKFB3-isoform 1, PFKFB3-isoform 2, PFKFB3-isoform 3, PFKFB3-isoform 4, PFKFB4, PFKL, PFKM, PFKP, PGAM1, PGAM2, PGAM4, PGK1, PGK2, PKL, PKM1, PKM2, PKR, TPI1

Lactate production and transporters:

LDHA, LDHB, LDHC, LDHAL6A, LDHAL6B, UEVLD, SLC16A1, SLC16A2, SLC16A3, SLC16A4, SLC16A5, SLC16A6, SLC16A7, SLC16A8, SLC16A9, SLC16A10, SLC16A11, SLC16A12

Gluconeogenesis:

BCAT1, BCAT2, FBP1, FBP2, G6PC, G6PC2, G6PC3, GPT, GPT2, LDHD, PC, PCK1, PCK2

Glycogen metabolism:

GBE1, GYS1, GYS2, PGM1, PGM2, UGP2

Hexosamine metabolism:

GFPT1, GFPT2, GNPNAT1, PGM3, UAP1, UAP1L1

Pentose phosphate pathway:

G6PD, H6PD, PGD, PGLS, PRPS1, PRPS1L1, PRPS2, RBKS, RPE, RPEL1, RPIA, TALDO1, TKT, TKTL1, TKTL2

Glycerol/fatty acid/cholesterol synthesis:

ACACA, ACACB, ACAT1, ACAT2, ACLY, ACSBG1, ACSBG2, ACSL1, ACSL3, ACSL4, ACSL5, ACSL6, ACSM1, ACSM2A, ACSM2B, ACSM3, ACSM4, ACSM5, FADS1, FADS2, FASN, GPD1, GPD1L, HMGCR, HMGCS1, HMGCS2, MLYCD, SCD, SCD5, SLC25A1, SLC27A2

Serine/ glycine/ one-carbon metabolism:

AHCY, AHCYL1, AHCYL2, AMT, BHMT, DHFR, DHFRL1, DLD, DNMT1, DNMT3A, DNMT3B, DNMT3L, GCSH, GLDC, MAT1A, MAT2A, MAT2B, MTHFD1, MTHFD1L, MTHFD2, MTHFD2L, MTHFR, MTR, PHGDH, PSAT1, PSPH, SHMT1, SHMT2

TCA cycle:

ACO1, ACO2, D2HGDH, DHTKD1, DLAT, DLD, DLST, FH, IDH1, IDH2, IDH3A, IDH3B, IDH3G, L2HGDH, MDH1, MDH1B, MDH2, OGDH, OGDHL, PDHA1, PDHA2, PDHB, PDHX, PDK1, PDK2, PDK3, PDK4, PDP1, PDP2, PDPR, SDHA, SDHAF1, SDHAF2, SDHAF3, SDHAF4, SDHB, SDHC, SDHD, SUCLA2, SUCLG1, SUCLG2, UEVLD

Glutamine transporters and glutaminolysis:

GLS2, GLS-isoform 1, GLS-isoform 2, GLS-isoform 3, GLUD1, GLUD2, GOT1, GOT2, SLC1A1, SLC1A2, SLC1A3, SLC1A4, SLC1A5, SLC1A6, SLC38A1, SLC38A3, SLC38A5, SLC38A7

Redox balance:

CBS, CTH, G6PD, GCLC, GCLM, GSR, GSS, IDH1, IDH2, ME1, ME2, ME3, MTHFD1, NNT, PGD, SLC7A11

GSH synthesis:

CBS, CTH, GCLC, GCLM, GSR, GSS, SLC7A11

Fatty acid oxidation:

AADAC, ABHD12, ABHD6, ACAA1, ACAA2, ACAD10, ACAD11, ACAD8, ACAD9, ACADL, ACADM, ACADS, ACADSB, ACADVL, ALDH1B1, ALDH2, ALDH3A2, ALDH7A1, ALDH9A1, CEL, CPT1A, CPT1B, CPT1C, CPT2, ECH1, ECHS1, ECI1, ECI2, EHHADH, ETFA, ETFB, HADH, HADHA, HADHB, HSD17B10, HSD17B4, LIPC, LIPE, LIPF, LIPG, MGLL, PAFAH1B1, PAFAH1B2, PAFAH1B3, PNLIP, PNLIPRP1, PNLIPRP2, PNLIPRP3, PNPLA2, PNPLA3, SCP2

Acetate metabolism:

ACOT12, ACSS1, ACSS2, ACSS3

Nucleotide metabolism:

ADA, ADCY1, ADCY10, ADCY2, ADCY3, ADCY4, ADCY5, ADCY6, ADCY7, ADCY9, ADSL, ADSS, ADSSL1, AK1, AK2, AK3, AK4, AK5, AK6, AK7, AK8, AK9, AMPD1, AMPD2, AMPD3, APRT, ATIC, CAD, CANT1, CDA, CECR1, CMPK1, CMPK2, CTPS1, DCK, DCTD, DHODH, DTYMK, DUT, GART, GDA, GMPS, GUCA1A, GUCA1B, GUCA1C, GUCA2A, GUCA2B, GUCD1, GUCY1A2, GUCY1A3, GUCY1B3, GUCY2C, GUCY2D, GUCY2F, GUK1, HPRT1, IMPDH1, IMPDH2, NME1, NME2, NME3, NME4, NME6, NME7, NT5C2, PAICS, PDE10A, PDE4D, PFAS, PNP, PPAT, RRM1, RRM2, RRM2B, TK1, TK2, TYMP, TYMS, UCKL1, UMPS, UPP1, UPP2, XDH

• Best coverage – Systematically covers the enzymatic and protein components in the core metabolic pathways and crucial metabolite transport systems
• Detailed annotation – Each metabolic gene is annotated with information of the functional protein or protein subunit by integrating genome/transcriptome data with protein UniProt debases.
• Representative transcript chosen – Detects mRNA transcript that encodes the canonical protein/subunit sequence in Uniprot debases
• Rigorous – All assays are elaborately optimized and rigorously validated 
• Convenient – Ready-to-use, accurate profiling, rapid results.

 

 

overview-protocol2

Compatible qPCR Instruments:
ABI ViiA™ 7
ABI 7500 & ABI 7500 FAST
ABI 7900HT
ABI QuantStudio™ 6 Flex Real-Time PCR system
ABI QuantStudio™ 7 Flex Real-Time PCR system
ABI QuantStudio™ 12K Flex Real-Time PCR System
Bio-Rad CFX384
Bio-Rad iCycler & iQ Real-Time PCR Systems
Eppendorf Realplex
QIAGEN Rotor Gene Q 100
Roche Light Cycler 480
Stratagene Mx3000
Roche Light Cycler 480