Arraystar G4 Cut&Tag is a cutting-edge technology designed to map and profile DNA G-quadruplex (dG4) structures in the genome at high resolution and specificity. To use the service, customers must first construct G4 Cut&Tag libraries with Arraystar G4 CUT&Tag Library Prep Kit. The constructed libraries are then sent to Arraystar for sequencing and data analysis service.
Place an order for Arraystar G4 CUT&Tag Library Prep Kit.
Benefits:
• High resolution, specificity, sensitivity, and reproducibility: High-resolution mapping of G4 sites within a few bases. Highly specific and sensitive antibody targeting for superior G4s signals. The results are highly reproducible across technical replicates.
• G4 detection in enhancer, promoter, and gene body regions, even for transient G4s in vivo.
• Convenience: Tagmentation is much easier to perform than ChIP-seq based methods. No optimization of fixation, sonication, digestion, or immunoprecipitation required.
• Improved sample prep: Cell samples are collected by centrifugation instead of original ConA magnetic beads, which is applicable to more sample types (e.g. most cell types or frozen tissues) at higher cell viability (> 85%).
DNA G-quadruplexes (dG4s) are non-canonical nucleic acid structures formed by guanine-rich sequences into four-stranded helices stabilized by guanine tetrads and monovalent cations (e.g., K ). G-quadruplexes play crucial roles in several key biological processes, including transcription regulation, telomere maintenance, and genome stability. G-quadruplexes have attracted attention for their links to diseases, including cancer, and neurodegenerative diseases.
G4 Cut&Tag (Cleavage Under Targets and Tagmentation) is a cutting-edge technology designed to map and profile DNA G-quadruplex (dG4) structures with high resolution and specificity. In the G4 Cut&Tag procedure, native G4 structures within mildly permeabilized cells are bound with G4-specific antibody BG4, also further stabilizing their conformation in vivo. A Protein A/G-Tn5 transposase complex is then attached to the antibody, which, upon activation with Mg²?, cleaves the DNA around G4 sites while simultaneously tagging the sequencing adapters (tagmentation). Unlike ChIP-seq, G4 CUT&Tag bypasses chromatin fragmentation (sonication), crosslinking, and immunoprecipitation, thereby greatly reducing background noise and increasing reliability.