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Only 5ug total RNA is required! Cover mRNA & LncRNA, or circRNA modifications.
Current transcriptome-wide RNA modification profiling methods deal mostly with mapping the modified sites but are unable to quantify the percentage of modified RNA for that transcript. The lack of such quantitative information has been a major concern for scientists [1,2].
Arraystar Epitranscriptomic Arrays combine the two-color channel microarray technology with RNA modification immunoprecipitation have the power to determine: which gene transcripts are modified, the modification quantity and the percentage of modification for each transcript (Table 1).
| “The modification quantity for each transcript” |
| |
Raw intensity |
|
m6A Quantity |
|
Annotations |
Gene
Symbol |
IP |
Input |
… |
IP(Corrected) |
… |
Seqname |
| GJD4 |
25.43 |
22.04 |
… |
13.85 |
… |
NM_153368 |
| NOP9 |
83.72 |
269.53 |
… |
76.98 |
… |
NM_174913 |
| TM7SF3 |
73.85 |
948.20 |
… |
46.71 |
… |
NM_016551 |
| RNF167 |
506.53 |
1150.78 |
… |
643.45 |
… |
NM_015528 |
| TMR10 |
25.24 |
22.23 |
… |
14.41 |
… |
NM_017762 |
|
| “The percentage of modification for each transcript” |
| |
Raw intensity |
|
m6A |
|
Annotations |
Gene
Symbol |
IP |
Input |
… |
%Modified |
… |
Seqname |
| GJD4 |
25.43 |
22.04 |
… |
45.1% |
… |
NM_153368 |
| NOP9 |
83.72 |
269.53 |
… |
15.3% |
… |
NM_174913 |
| TM7SF3 |
73.85 |
948.20 |
… |
3.2% |
… |
NM_016551 |
| RNF167 |
506.53 |
1150.78 |
… |
15.8% |
… |
NM_015528 |
| MTMR10 |
25.24 |
22.23 |
… |
44.5% |
… |
NM_017762 |
|
Table 1. Epitranscriptomic Array detects the modified RNA in Cy5 and unmodified RNA in Cy3 channels on the same array, such that the modified percentages for each transcript can be measured. “The modification quantity” is calculated based on the normalized IP intensities, and “The percentage of modification” is calculated as IP/Total RNA.
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