Seq-Star™ Rapid RNA-seq Kit (Illumina) is a perfect kit for rapid construction of stranded RNA-seq library from as little as 100 pg rRNA-depleted or oligo(dT)-enriched RNA. The template-switching directly incorporates adapter sequences without the needs for finicky 2nd cDNA synthesis, end repair, A-addition and adapter ligation as required in the classic methods, reducing the time, costs and bias while increasing the prep throughput. 
From RNA sample to sequencing library, simple and easy in short 3.5 hours! 

Perfect kit for RNA-seq library prep
Seq-Star™ Rapid RNA-seq Kit is better than classic RNA-seq prep kits in simplicity, speed, low input RNA amount requirement, robustness and uncompromising results.
Table 1.  Characteristics of the RNA-seq preps

* Classic dUTP-based RNA-seq kits such as Illumina TruSeq Stranded mRNA Library Preparation Kit, KAPA Stranded mRNA-Seq Kit, and others.

Optimized prep reactions
The reagent formulation and reaction conditions are highly optimized to produce high yield, high quality libraries without adapter dimers and erratic size distribution (Fig.2).

Figure 2. Agilent 2100 Bioanalyzer analysis of the RNA-seq library prepared using Seq-Star™ Rapid RNA-seq kit, showing the high yield, steady size distribution, and the absence of adapter dimers.

Robust performance and uncompromising results
Table 2. Sequencing read stats

High technical reproducibility
Technically replicated library preps show excellent reproducibility at R² > 0.99.

Figure 3. FPKM scatter plot of the technically replicated library preps generated by Seq-Star™ Rapid RNA-seq Kit.

Seq-Star™ Rapid RNA-seq Kit (Illumina) Workflow
From rRNA depleted or oligo(dT)-enriched RNA to stranded RNA-seq library

 Manual Seq-Star™ Rapid RNA-seq kit (Illumina)V2