Print |

You have not viewed any products recently.

 

tRNA, tRF and tiRNA Research​

How to Achieve High tRNA cDNA Synthesis Efficiency?

 

tRNAs undergo by far the greatest number of and the most chemically diverse post-transcriptional modifications, which badly affect tRNA cDNA synthesis efficiency. Methylation, one of the most prevalent modifications, is the main obstacle that hinders transcriptional elongation and thus leads to non-effective cDNA conversion and qPCR. By using an excellent RNA demethylase, Arraystar developed rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit (Cat# AS-FS-004), a highly efficient system that removes methylations on the RNA to obtain a new level of accuracy on the tRNA detection.

Figure_1-webFigure_2-web

Figure 1. tRNA demethylation treatment dramatically increased PCR assay performance. Upper: tRNA levels in 3 pancreatic carcinomas and 3 matched para-carcinoma RNA samples with or without the RNA demethylation treatment. Error bars indicate SEM (n=3). De: demethylation, Un: untreated with demethyation. Bottom: Principle component analysis (PCA) analysis of tRNA profiles for the pancreatic carcinoma and para-carcinoma tissues. The Cancer (Demethylation) and Para-carcinoma (Demethylation) samples are well separated, whereas the Cancer and Para-carcinoma samples without the demethylation treatment are indistinguishable.


Related Products

rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit
nrStar™ tRNA PCR Arrays

Back to news

Publications >>

Circular RNAs as biomarkers in liquid biopsy in colorectal cancer. Valladares-Ayerbes M, et al.  Journal of Clinical Oncology, 2020​Full text>>

Defining the adult neural stem cell niche proteome identifies key regulators of adult neurogenesis. Kjell J, et al. Cell Stem Cell, 2020
Full text>>

 

Promotion >>

LncRNA Array Service
- Unparalleled accuracy, newest databases and dedicated annotations

Promo: 20% OFF

Learn more>>

 

Brochures >> 

Arraystar Epitranscriptomic Microarrays
- Quantifying the percentage of modifications

Epitranscriptomic_arrays-1

Download.pdf

Arraystar LncRNA Arrays
- New Versions Released in 2019

LncRNA_Arrays_-_Released_in_2019

Download.pdf

 

Webinar >>

Quantify the percentage of modification at transcript level
- Arraystar Epitranscriptomic Arrays

m6A_microarray_slideshow

Watch video

Arraystar LncRNA Microarray profiling
-
The wise choice!


Watch video

The only practical choice for circular RNA profiling

Watch video