| Unravel the tRNA pool alteration in diseases.
 Transfer RNAs (tRNAs) are ubiquitous and the most abundant of all small non-coding RNA molecules. As a fundamental component in translation, tRNAs serve as the physical link between the mRNA and the protein sequences.
A wide variety of biological processes such as cell proliferation differentiation and apoptosis are always accompanied with tRNA level variation [1-3]. Many diseases, for example, type 2 diabetes[4], Huntington's disease[5], and HIV infection[6], are impacted by altered tRNA pools. Dysregulated tRNA repertoire can promote tumorigenesis and cancer progression[7-8]. tRNA repertoire has become an important source of information in the study of codon usage, protein translation efficiency and accuracy, biological processes and human diseases. Learn more>
To conveniently, reliably and accurately profile the tRNA repertoire, Arraystar has developed the first commercially available nrStar™ Human tRNA Repertoire PCR Array in the market. The PCR Array profiles 66 PCR-distinguishable nuclear tRNAs and all human mitochondrial tRNAs, covering all anti-codons provided by GtRNAdb and tRNAdb databases. Specially optimized tRNA demethylation is used to overcome heavy tRNA modifications to gain upmost profiling efficiency.
Highlights
- Profile nuclear and mitochondrial tRNAs of all anti-codons in the GtRNAdb and tRNAdb databases
- Powerful companion tRNA demethylation to achieve efficient tRNA profiling
- Fully experimentally validated primers across numerous tissues and cell lines
- Easy-to-use convenient panel to obtain results in hours
References
1. Gingold H. et al. (2014) "A dual program for translation regulation in cellular proliferation and differentiation." Cell 158(6):1281-92 [PMID: 25215487]
2. Pavon-Eternod M. et al. (2013) "Overexpression of initiator methionine tRNA leads to global reprogramming of tRNA expression and increased proliferation in human epithelial cells." RNA 19(4):461-6 [PMID: 23431330]
3. Mei Y. et al. (2010) "Apoptotic regulation and tRNA." Protein Cell 1(9):795-801 [PMID: 21113408]
4. Krokowski D. et al. (2013) "A self-defeating anabolic program leads to beta-cell apoptosis in endoplasmic reticulum stress-induced diabetes via regulation of amino acid flux." J. Biol. Chem. 288(24):17202-13 [PMID: 23645676]
5. Girstmair H. et al. (2013) "Depletion of cognate charged transfer RNA causes translational frameshifting within the expanded CAG stretch in huntingtin." Cell Rep 3(1):148-59 [PMID: 23352662]
6. van Weringh A. et al. (2011) "HIV-1 modulates the tRNA pool to improve translation efficiency." Mol. Biol. Evol. 28(6):1827-34 [PMID: 21216840]
7. Berns A. (2008) "A tRNA with oncogenic capacity." Cell 133(1):29-30 [PMID: 18394985]
8. Goodarzi H. et al. (2016) "Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression." Cell 165(6):1416-27 [PMID: 27259150]
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