Arraystar R-loop Cut&Tag Service is a cutting-edge technology designed to map and profile R-loop structures in the genome at high resolution and specificity. To use the service, customers must first construct R-loop Cut&Tag libraries with Arraystar R-Loop CUT&Tag Library Prep Kit. The constructed libraries are then sent to Arraystar for sequencing and data analysis service.
Place an order for Arraystar R-Loop CUT&Tag Library Prep Kit.
Benefits:
• High resolution, specificity, sensitivity, and reproducibility: High-resolution mapping of R-loop sites within a few bases. Highly specific and sensitive antibody targeting for superior R-loop signals. The results are highly reproducible across technical replicates.
• R-loop detection in enhancer, promoter, and gene body regions, even for transient low abundance R-loops in vivo.
• Convenience: Tagmentation is much easier to perform than immunoprecipitation based methods. No optimization of sonication, digestion, or immunoprecipitation required.
• Improved sample prep: Cell samples are collected by centrifugation instead of ConA magnetic beads in the original protocol, which is applicable to more sample types (e.g. most cell types or frozen tissues) at higher cell viability (> 85%).
R-loops are RNA:DNA three-stranded hybrid structures that widely exist in the genome. They participate in many biological processes, playing particularly important roles in gene regulation and genome stability.
Arraystar Rapid R-loop CUT&Tag uses pre-assembled transposomes containing hyperactive protein A/Tn5 transposase fusion enzyme and transposons to target S9.6 antibody bound R-loops and tagment the R-loop sites (CUT&Tag, Cleavage Under Targets and Tagmentation)(Fig.1). Compared with R-loop profiling by DNA:RNA immunoprecipitation (DRIP-seq), R-loop CUT&Tag-seq vastly simplifies the R-loop sequencing library prep, allowing lower sample inputs, higher resolution, lower background noise, reliable signals, fast and better results.
Fig.1. Arraystar R-loop CUT&Tag uses transposase/protein A fusion to target and tagment R-loops via S9.6 antibody.