R-Loop CUT&Tag Library Prep Kit

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Arraystar R-loop CUT&Tag Library Prep Kit is designed for constructing sequencing library to map and profile R-loops genome-wide. For the constructed libraries, Arraystar offers sequencing and bioinformatics analysis service.

Benefits:
• High resolution, specificity, sensitivity, and reproducibility: High-resolution mapping of R-loop sites within a few bases. Highly specific and sensitive antibody targeting for superior R-loop signals. The results are highly reproducible across technical replicates.
• R-loop detection in enhancer, promoter, and gene body regions, even for transient low abundance R-loops in vivo.
• Convenience: Tagmentation is much easier to perform than immunoprecipitation based methods. No optimization of sonication, digestion, or immunoprecipitation required.
• Improved sample prep: Cells are collected by centrifugation instead of ConA magnetic beads in the original protocol, which is applicable to more sample types (e.g. most cell types or frozen tissues) at higher cell viability (> 85%).

Product NameCatalog NoSizePrice
R-Loop CUT&Tag Library Prep Kit AS-TN-005 12 reactions
$1,098.00
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R-loop is a special three-stranded RNA:DNA:DNA hybrid structure widely exists in the genome. R-loops participate in many biological processes and play an important role in genome instability.

Arraystar R-loop CUT&Tag Library Prep Kit is designed for constructing sequencing library to map and profile R-loops genome-wide. In the CUT&Tag (Cleavage Under Targets & Tagmentation), S9.6 antibody is used to bind R-loops in the genomic DNA. The antibody bound R-loop sites are then fragmented and transposon tagged by protein A-Tn5 transposase fusion enzyme in a single tagmentation step. This method can replace traditional immunoprecipitation based sequencing (e.g. DRIP-seq) due to its low background, high signal-to-noise ratio, better reproducibility, and ease of use.

As an example, the R-loop CUT&Tag library was prepared using 200,000 HEK293T cells with the kit according to the kit protocol. The sequencing results are displayed in Fig.1.

R-loop_cut_tag_kit_-_1

Fig.1. The R-loop peak distribution in standardized transcription unit, showing significant enrichment in promoter region (TSS).