Epigenetic modifications play critical roles in gene expression regulation, and have been associated with the induction and maintenance of various human cancers. The common epigenetic changes include DNA methylation, histone modifications and chromatin remodeling, which are mediated by the enzymes that chemically modify genomic DNA, histones, and chromatin remodeling factors. LncRNAs have emerged as novel epigenetic modulators, and might participate in epigenetic signaling pathway by targeting the critical epigenetic modification enzymes and protein factors.
The LncPath™ Human Epigenetic Pathway LncRNA Microarray simultaneously profiles the expression of 1,321 LncRNAs and 477 their potential coding targets related to the epigenetic signaling pathway. The LncPath™ Mouse Epigenetic Pathway LncRNA Microarray simultaneously profiles the expression of 884 LncRNAs and 835 their potential coding targets related to the epigenetic signaling pathway. The LncRNAs whose gene loci located at or near the protein-coding genes critical in the epigenetic pathway, and LncRNAs which have high possibility as the potential ceRNAs of the key epigenetic genes, are carefully selected from the authoritative databases by using a rigorous process. By limiting LncRNAs to those most relevant to the epigenetic pathway, analysis can be achieved quickly and precisely due to a highly specific yet smaller data set. More importantly, it can establish the expression relationship between LncRNAs and their potential protein-coding targets involved in the epigenetic signaling pathway, thereby providing comprehensive insights into the underlying regulatory mechanisms of LncRNAs in epigenetic modifications.
• Reliable epigenetic pathway focus LncRNA collection
• Simultaneous analysis of LncRNAs and their potential coding gene targets related to the epigenetic pathway
• Efficient and robust labeling system
• Innovative probe design
• Guaranteed performance
An example showing a protein coding gene and its corresponding regulatory LncRNAs
Click the LncRNA accession number listed in databases, you will see the figures showing the detailed information about the LncRNAs and their potential target gene.
Figure 1. An example of genomic map views of the LncRNA ENST00000580587 whose gene is located at the BRD2 gene locus. From top to bottom,
Genome view: A chromosome ideogram showing the map position of the LncRNA ENST00000580587 and its potential target gene BRD2 (red bar).
Map view ruler: The map coordinates of the human genome assembly hg19 for the map views below.
LncRNA map view: The LncRNAs whose genes located at or near the BRD2 gene are presented in the Noncoding panel (shaded blue). The LncRNAs are indicated by the transcript IDs, the exons by solid blocks, the introns by thin lines, and the transcription directions by arrows.
Coding gene map view: The coding gene BRD2 is presented in the Coding panel (shaded green). The coding gene is indicated by its canonical transcript ID, the exons by solid blocks, the introns by thin lines, and the transcription direction by arrows.
Figure 2. The relationship between LncRNA ENST00000580587 and its protein coding gene target BRD2. The other neighboring LncRNAs which may regulate BRD2 expression are also shown.
Figure 3. The LncRNA uc011kzr.2 may function as a competing endogenous RNA (ceRNA) of the protein coding gene BRD2.
* MuTaMe Score, Mutually Targeted MRE Enrichment Score .
References1. Tay, Y., et al., Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs. Cell, 2011. 147(2): p. 344-57.