LC-MS Based mRNA Modification Analysis Service

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Arraystar LC-MS mRNA Modification Analysis Service analyzes 55 nucleoside modifications and characterizes global modification profile of mRNAs as mRNA biochemical properties vital to mRNA biogenesis, structure, functioning and implication in diseases.

Benefits

• Quantitative analysis of complex mRNA modifications from total RNA samples.
• Full service sample-to-data - From sample QC, mRNA enrichment, nucleoside analyte preparation, LC-MS/MS data acquisition, analysis to report.
• High Performance - Highly optimized experimental procedures, state-of-the-art LC-MS system, expertise in operation.
• Best analytical coverage - Simultaneous profiling of 55 nucleoside modifications in mRNAs.

How to Order

1. Check if your modifications of interest are in our standard detection list. 
2. If yes, please click "Request Quote" below, and our technical sales team will follow up shortly.
3. If not, contact support@arraystar.com to discuss customization options. (Additional costs may apply.)

Service NameCatalog NoSizePrice
LC-MS Based mRNA Modification Analysis Service - Eukaryotic AS-LC-m-S 1 sample
Total RNA Extraction Service AS-RE-S 1 sample

Table1. Nucleoside modifications profiled by Arraystar LC-MS mRNA Modification Analysis

Number Nucleoside Symbol Number Nucleoside Symbol
1 3'-O-methyladenosine 3'-OMeA 2 3'-O-methyluridine 3'-OMeU
3 2'-O-methylcytidine Cm 4 5-methyl-2-thiouridine m5s2U
5 3-methylcytidine m3C 6 5-methoxyuridine mo5U
7 5-methylcytidine m5C 8 pseudouridine
9 N6-isopentenyladenosine i6A 10 2'-O-methylinosine Im
11 5,2'-O-dimethylcytidine m5Cm 12 3-methyluridine m3U
13 1-methyladenosine m1A 14 1-methylpseudouridine m1
15 2-thiocytidine s2C 16 5-hydroxymethylcytidine hm5C
17 N2, N2, 7-trimethylguanosine m2,2,7G 18 5,2'-O-dimethyluridine m5Um
19 N4-acetyl-2'-O-methylcytidine ac4Cm 20 N6-threonylcarbamoyladenosine t6A
21 N6-methyladenosine m6A 22 2-methylthio-N6-threonylcarbamoyladenosine ms2t6A
23 3'-O-methylcytidine 3'-OmeC 24 5-carboxymethyluridine cm5U
25 2'-O-methyladenosine Am 26 5-methoxycarbonylmethyl-2-thiouridine mcm5s2U
27 N2, N2-dimethylguanosine m22G 28 5-Methoxycarbonylmethyluridine mcm5U
29 5'-O-methylthymidine 5'-OMeT 30 2-methylthio-N6-isopentenyladenosine ms2i6A
31 2'-O-methyluridine Um 32 Peroxywybutosine o2w
33 inosine I 34 5-taurinomethyl-2-thiouridine tm5s2U
35 2'-O-methylguanosine Gm 36 5-oxyacetic acid uridine cmo5U
37 1-methylguanosine m1G 38 5-carbamoylmethyuridine ncm5U
39 7-methylguanosine m7G 40 Queuosine Q
41 N2-methylguanosin m2G 42 5-taurinomethyluridine tm5U
43 3'-O-methylinosine 3'-OMeI 44 5-formyl-2'-O-methylcytidine f5Cm
45 2-thiouridine s2U 46 dihydrouridine D
47 4-thiouridine s4U 48 5-formylcytidine f5c
49 5-methyluridine m5U 50 wybutosine W
51 N4-acetylcytidine ac4C 52 5-methoxycarbonylmethyl-2'-o-methyluridine mcm5Um
53 N6, O2'-methyladenosine m6Am 54 5-methylaminomethyl-2-thiouridine mnm5s2U
55 5-hydroxyuridine ho5U      

Deciphering the interplay between SETD2 mediated H3K36me3 and RNA N6-methyladenosine in clear cell renal cell carcinoma (ccRCC). Shafiq Shaikh, et al. Epigenetics, 2025

Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells. Datta C,et al. Science Advances, 2022