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Seq-Star™ Stranded RNA-seq Kit (Illumina, setA)
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Seq-Star™ Stranded RNA-seq Kit (Illumina, setB)
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Seq-Star™ Stranded RNA-seq Kit (Illumina) includes all the reagent and enzyme components for constructing stranded RNA-seq libraries starting from 10 ~ 400 ng of rRNA-depleted or oligo(dT)-enriched RNA. The RNA-seq library constructed with the kit is excellent for the transcriptome of both mRNAs and lncRNAs and is fully compatible with Illumina sequencing platforms.
The Kit uses dUTP incorporation to mark the cDNA synthesis strand and generates sequencing reads only from the transcribed strand direction of the double stranded DNA genome (Fig. 1). Many long non-coding RNAs (lncRNA) can be transcribed in either sense- or antisense directions in reference to the nearby mRNA genes having differing regulatory functions, the high strand specificity is valuable for profiling the entire transcriptome including both mRNA and lncRNA classes.
Specially designed library PCR amplification system produces unbiased, high fidelity libraries to maximize the uniformity of read coverage along the entire transcripts and to faithfully represent the relative RNA abundance levels (Fig. 1).
Figure 1. Arraystar Seq-Star™ Stranded RNA-seq Kit produces libraries at extremely high strand specificity that distinguishes the RNA transcript strand directions (red or blue arrows). The sequencing read coverage along the transcript is unbiased and uniform, which greatly enhances the quantification accuracy.
• State-of-the-art techniques throughout the library construction workflow.
• Easily controlled RNA fragmentation with divalent metal cation at high temperature to eliminate the needs for sonication and the equipment.
• Stranded dUTP incorporation to ensure extremely high transcript strand specificity.
• Unbiased, high fidelity PCR amplification for uniform read coverage and accurate quantification.
• Single reagent mix in each reaction step for simplicity and consistency.
• Companion Seq-Star™ DNA Size Selection Beads Kit for convenient library sizing and maximal cDNA recovery compared with gel electrophoresis.
• Excellent for RNA-seq of transcriptome of both mRNAs and long non-coding RNAs.