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rtStar™ Pre-designed tRF&tiRNA Primer Sets AS-NR-002-1-M 100 reactions $68.00
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rtStar™ Pre-designed tRF&tiRNA Primer Sets are part of the nrStar™ tRF&tiRNA PCR System, and can be used in combination with rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor) for mature tRF&tiRNA detection and quantification. They cover 185 prevalent tRFs & tiRNAs compiled in the databases and newly reported in recent publication. All the primers have been validated in serum samples, and guarantee the most accurate and specific performance when used with Arraystar SYBR Green qPCR Master Mix (Figure 1).

tRF_tiRNA_primer2

Figure 1. Amplification curve (A) and Dissociation curve (B) of example tRF-5005A.

• Reliable results with validated primers
• High sensitivity and specificity
• Accurate quantification over a wide linear range
• Time and cost savings with validated primers and SYBR Green assay

 

tRF&tiRNA name tRF sequence

Source tRNA

Catalog Number
3'tiR_007_GluTTC (n) TTTCACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACCA GluTTC (n) AS-NR-002-1-001
3'tiR_012_ArgCCT (n) CCTAAGCCAGGGATTGTGGGTTCGAGTCCCACCCGGGGTACCA ArgCCT (n) AS-NR-002-1-002
3'tiR_026_GlnCTG (n) TGAATCCAGCGATCCGAGTTCAAATCTCGGTGGAACCTCCA GlnCTG (n) AS-NR-002-1-003
3'tiR_028_HisGTG (mt) TGTGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCC HisGTG (mt) AS-NR-002-1-004
3'tiR_037_ArgCCG (n) GGAGCTGGGGATTGTGGGTTCGAGTCCCATCTGGGTCGCCA ArgCCG (n) AS-NR-002-1-005
3'tiR_056_ValTAC (mt) TACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCA ValTAC (mt) AS-NR-002-1-006
3'tiR_060_MetCAT (n) TCATAATCTGAAGGTCGTGAGTTCGATCCTCACACGGGGCACCA MetCAT (n) AS-NR-002-1-007
3'tiR_063_ArgCCT (n) TCCTAAGCCAGGGATTGTGGGTTCGAGTCCCATCTGGGGTGCCA ArgCCT (n) AS-NR-002-1-008
3'tiR_075_GluTTC (mt)
GluTTC (mt-la)
TTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACC

GluTTC (mt)
GluTTC (mt-la)

AS-NR-002-1-009
3'tiR_078_ArgTCT (n) TTCTAATTCAAAGGTTCCGGGTTCGAGTCCCGGCGGAGTCGCCA ArgTCT (n) AS-NR-002-1-010
3'tiR_080_ProTGG (mt) TTGGGTGCTAATGGTGGAGTTAAAGACTTTTTCTCTGACC ProTGG (mt) AS-NR-002-1-011
3'tiR_082_ThrTGT (mt) TTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACACCA ThrTGT (mt) AS-NR-002-1-012
3'tiR_088_LysCTT (n) CTTAATCTCAGGGTCGTGGGTTCGAGCCCCACGTTGGGCGCCA LysCTT (n) AS-NR-002-1-013
5003/4C GCATGGGTGGTTCAGTGGTAGAATTCTCGCC
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC
GlyGCC
GlyGCC
AS-NR-002-1-014
5008C GCGTTGGTGGTATAGTGGTGAGCATAGCTG GlyTCC AS-NR-002-1-015
5009C GCTTCTGTAGTGTAGTGGTTATCACGTTCGC ValCAC AS-NR-002-1-016
5016C GGGGGTATAGCTCAGTGGTAGAGCATTTGACT CysGCA AS-NR-002-1-017
5026/27C GTTTCCGTAGTGTAGTGGTCATCACGTTCGC
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC
ValAAC
ValAAC
ValCAC
AS-NR-002-1-018
TRF62 AGCAGAGTGGCGCAGCGGAAGCGTGCTGG MetCAT AS-NR-002-1-019
TRF315 GCCCGGCTAGCTCAGTCGGTAGAGCATGG LysCTT AS-NR-002-1-020
TRF327 GCCTTGGTGGTGCAGTGGTAGAATTCTCGCCT GlyCCC AS-NR-002-1-021
TRF353 GGCCCCATGGTGTAATGGTTAGCACTCTGGA GlnTTG AS-NR-002-1-022
TRF419 GGTAGTGTGGCCGAGCGGTCTAAGGCGCTG LeuTAG AS-NR-002-1-023
tiRNA-5033-ProTGG-1 GGCTCGTTGGTCTAGGGGTATGATTCTCGGTTT ProTGG AS-NR-002-1-024
tiRNA-5033-GluTTC-1 TCCCACATGGTCTAGCGGTTAGGATTCCTGGTT GluTTC AS-NR-002-1-025
tiRNA-5029-GlyGCC-2 GCGCCGCTGGTGTAGTGGTATCATGCAAG GlyGCC AS-NR-002-1-026
tiRNA-5034-ValCAC-2 GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTC ValCAC AS-NR-002-1-027
tiRNA-5034-ValCAC-3 GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTC ValCAC AS-NR-002-1-028
tiRNA-5030-HisGTG-1 GCCGTGATCGTATAGTGGTTAGTACTCTGC HisGTG AS-NR-002-1-029
tiRNA-5030-GluTTC-1 TCCCACATGGTCTAGCGGTTAGGATTCCTG GluTTC AS-NR-002-1-030
tiRNA-5033-GluTTC-2 TCCCATATGGTCTAGCGGTTAGGATTCCTGGTT GluTTC AS-NR-002-1-031
tiRNA-5030-LysCTT-2 GCCCGGCTAGCTCAGTCGGTAGAGCATGGG LysCTT AS-NR-002-1-032
tiRNA-5029-ProAGG GGCTCGTTGGTCTAGGGGTATGATTCTCG ProAGG AS-NR-002-1-033
tiRNA-5031-GluTTC-1 TCCCTGGTGGTCTAGTGGCTAGGATTCGGCG GluTTC AS-NR-002-1-034
tiRNA-5031-HisGTG-1 GCCGTGATCGTATAGTGGTTAGTACTCTGCG HisGTG AS-NR-002-1-035
tiRNA-5031-GluCTC-1 TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG GluCTC AS-NR-002-1-036
tiRNA-5034-GlyCCC-1 GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCC GlyCCC AS-NR-002-1-037
tiRNA-5034-GluTTC-1 TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTT GluTTC AS-NR-002-1-038
tiRNA-5033-LysTTT-1 GCCCGGATAGCTCAGTCGGTAGAGCATCAGACT LysTTT AS-NR-002-1-039
tiRNA-5032-LysTTT-1 GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC LysTTT AS-NR-002-1-040
tiRNA-5031-PheGAA GCCGAAATAGCTCAGTTGGGAGAGCTTTAGA PheGAA AS-NR-002-1-041
tiRNA-5034-ValTAC-3 GGTTCCATAGTGTAGTGGTTATCACATCTGCTTT ValTAC AS-NR-002-1-042
tiRNA-5030-GlnTTG-3 GGTCCCGTGGTGTAATGGTTAGCACTCTGG GlnTTG AS-NR-002-1-043
tiRNA-5029-GlyGCC-3 GCATTGGTGGTTCAGTGGTAGAATTCTCG GlyGCC AS-NR-002-1-044
tiRNA-5035-GluTTC-1 TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTT GluTTC AS-NR-002-1-045
tiRNA-5035-GluTTC-2 TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTT GluTTC AS-NR-002-1-046
tiRNA-5034-GluTTC-2 TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTT GluTTC AS-NR-002-1-047
tiRNA-5035-GluTTC-3 TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTTT GluTTC AS-NR-002-1-048
tiRNA-5032-GluTTC-1 TCCCACATGGTCTAGCGGTTAGGATTCCTGGT GluTTC AS-NR-002-1-049
tiRNA-5035-GluCTC TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCT GluCTC AS-NR-002-1-050
tiRNA-5030-SerGCT-3 GACGAGGTGGCCGAGTGGTTAAGGCGATGG SerGCT AS-NR-002-1-051
tiRNA-5030-SerGCT-1 GACGAGGTGGCCGAGTGGTTAAGGCAATGG SerGCT AS-NR-002-1-052
tiRNA-5031-HisGTG-2 GCCGTAATCGTATAGTGGTTAGTACTCTGCG HisGTG AS-NR-002-1-053
tiRNA-5029-AlaAGC-1 GGGGGATTAGCTCAAATGGTAGAGCCCTC AlaAGC AS-NR-002-1-054
tiRNA-5032-LysCTT-1 GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC LysCTT AS-NR-002-1-055
1001 GAAGCGGGTGCTCTTATTTT SerTGA AS-NR-002-1-056
1003 GCTAAGGAAGTCCTGTGCTCAGTTTT SerGCT AS-NR-002-1-057
1004 GTGTGTAGCTGCACTTTT AspGTC AS-NR-002-1-058
1005 ATGTGGTGGCTTACTTT SerGCT AS-NR-002-1-059
1006 GTGTAAGCAGGGTCGTTTT ArgACG AS-NR-002-1-060
1007 TTCAAAGGTGAACGTTT GlnTTG AS-NR-002-1-061
1010 GGTGTGGTCTGTTGTTT ValTAC AS-NR-002-1-062
1012 GCACGAAAATGTGTTTT GlyGCC AS-NR-002-1-063
1013 GGCGATCACGTAGATTTT AlaCGC AS-NR-002-1-064
1015 TGTGCTCCGGAGTTACCTCGTTT CysGCA AS-NR-002-1-065
1020 GAGAGCGCTCGGTTTTT PheGAA AS-NR-002-1-066
1025 GAGGGTTCTCACCTTCTCTCTCCGATTT IleAAT AS-NR-002-1-067
1026 TTCCGTGGGTTTGTTTT IleAAT AS-NR-002-1-068
1027 ATGGCCGCATATATTT MetCAT AS-NR-002-1-069
1028 GAGGCTTAAACTTTT AspGTC AS-NR-002-1-070
1029 ATAGGTATTAAGGTTTT AlaTGC AS-NR-002-1-071
1030 GGAATGTCAGCTTTT SerAGA AS-NR-002-1-072
1031 ACAAGTGCGGTTTTTT TyrGTA AS-NR-002-1-073
1032 AAGAGGAGTTGTTTT LeuTAA AS-NR-002-1-074
1033 GTGGGGTGCCTCACAGCTTCGCTGCGTGAGCATTTT ArgACG AS-NR-002-1-075
1035 GATATCCAACCTTCGGCTATAGGGTGGAGACTTTTT ThrCGT AS-NR-002-1-076
1036 GGGTTGCTGTCTTTT LeuAAG AS-NR-002-1-077
1037 ACCTCAGAAGGTCTCACTTT LeuTAG AS-NR-002-1-078
1038 AGGTGAAAGTTCCTTT ArgCCT AS-NR-002-1-079
1039 TCGAGAGGGGCTGTGCTCGCAAGGTTTCTTT ArgCCT AS-NR-002-1-080
1040 GTGGGTGGCTTTTTT IleAAT AS-NR-002-1-081
1041 TGCGGTACCACTTTT GlyTCC AS-NR-002-1-082
1042 AACCGAGCGTCCAAGCTCTTTCCATTTT ThrAGT AS-NR-002-1-083
3002B TCAAATCCCGGACGAGCCCCCA ProCGG AS-NR-002-1-084
3004B TCAAATCTCGGTGGGACCTCCA GlnTTG AS-NR-002-1-085
3006B TCAAGTCCCTGTTCGGGCGCCA LysTTT AS-NR-002-1-086
3031B TCGCTGGTTCGAATCCGGCTCGGAGGACCA TyrGTA AS-NR-002-1-087
3033A CCCACCCAGGGACGCCA AsnGTT AS-NR-002-1-088
3030A TTCCGGCTCGAAGGACCA TyrGTA AS-NR-002-1-089
3030B TCGATTCCGGCTCGAAGGACCA TyrGTA AS-NR-002-1-090
3002A ATCCCGGACGAGCCCCCA ProAGG AS-NR-002-1-091
3003A TCCGGGTGCCCCCTCCA CysGCA AS-NR-002-1-092
3003B TCAAATCCGGGTGCCCCCTCCA CysGCA AS-NR-002-1-093
3006A TCCCTGTTCGGGCGCCA LysTTT AS-NR-002-1-094
3008A ACCGGGCGGAAACACCA ValAAC AS-NR-002-1-095
3008B TCGAAACCGGGCGGAAACACCA ValAAC AS-NR-002-1-096
3009B TCGAACCCCACTCCTGGTACCA LeuTAA AS-NR-002-1-097
3011/12A ATCCCACTCCTGACACCA
ATCCCACTTCTGACACCA
LeuCAG
LeuCAA
LeuCAG
AS-NR-002-1-098
3016/18/22B TCGAGCCCCACGTTGGGCGCCA
TCGAGCCTCAGAGAGGGCACCA
LysCTT
MetCAT
AS-NR-002-1-099
3017A AGCCCCAGTGGAACCACCA ValTAC AS-NR-002-1-100
3017B TCGAGCCCCAGTGGAACCACCA ValTAC AS-NR-002-1-101
3019/20/21B TCGATCCCCAGTACCTCCACCA
TCGATCCCCGGCACCTCCACCA
TCGATCCCCGGCATCTCCACCA
AlaAGC
AlaTGC
AlaCGC
AlaTGC
AS-NR-002-1-102
3026B TCGATTCCCGGCCAACGCACCA GlyTCC AS-NR-002-1-103
3027/28B TCGATTCCCGGCCCATGCACCA
TCGATTCCCGGCCAATGCACCA
GlyGCC
GlyGCC
AS-NR-002-1-104
3019A TCCCCAGTACCTCCACCA AlaAGC AS-NR-002-1-105
3020/21A TCCCCGGCACCTCCACCA
TCCCCGGCATCTCCACCA
AlaTGC
AlaAGC
AlaTGC
AlaCGC
AS-NR-002-1-106
3022A TCCCCGTACGGGCCACCA IleAAT AS-NR-002-1-107
3026/27/28A TTCCCGGCCAACGCACCA
TCCCGGCCAATGCACCA
TCCCGGCCCATGCACCA
GlyTCC
GlyCCC
GlyGCC
GlyGCC
AS-NR-002-1-108
3029A TTCCCGGTCAGGGAACCA GluCTC AS-NR-002-1-109
3031A TCCGGCTCGGAGGACCA TyrGTA AS-NR-002-1-110
5001A CCTTCGATAGCTCAG TyrGTA AS-NR-002-1-111
5001B CCTTCGATAGCTCAGCTGGTAGAGC TyrGTA AS-NR-002-1-112
5002A GACCCAGTGGCCTA ArgCCG AS-NR-002-1-113
5002B GACCCAGTGGCCTAATGGA ArgCCG AS-NR-002-1-114
5008B GCGTTGGTGGTATAGTGGTGAGC GlyTCC AS-NR-002-1-115
5009A GCTTCTGTAGTGTAG ValCAC AS-NR-002-1-116
5009B GCTTCTGTAGTGTAGTGGTTATC ValCAC AS-NR-002-1-117
5010A GGCCGGTTAGCTCAG SerACT AS-NR-002-1-118
5011A GGCTCGTTGGTCTAG ProCGG AS-NR-002-1-119
5012B GGCTCGTTGGTCTAGGGGTATGA ProCGG AS-NR-002-1-120
5013B GGCTCGTTGGTCTAGGGGTAT ProCGG AS-NR-002-1-121
5015/17A GGGGGTATAGCTC
GGGGGTGTAGCTC
AlaAGC
TyrGTA
ValAAC
CysGCA
AlaTGC
AlaCGC
AlaAGC
AS-NR-002-1-122
5016A GGGGGTATAGCTCAG CysGCA AS-NR-002-1-123
5019A GGTAGCGTGGCCGAGC LeuTAG AS-NR-002-1-124
5019B GGTAGCGTGGCCGAGCGGTCTAAG LeuTAG AS-NR-002-1-125
5020/21A GGTTCCATAGTGTA
GGTTCCATGGTGTA
ValTAC
GlnCTG
AS-NR-002-1-126
5020B GGTTCCATAGTGTAGTGGTTATC ValTAC AS-NR-002-1-127
5021B GGTTCCATGGTGTAATGG GlnCTG AS-NR-002-1-128
5022A GTAGTCGTGGCCGA SerTGA AS-NR-002-1-129
5022B GTAGTCGTGGCCGAGTGGTTAAGGC SerTGA AS-NR-002-1-130
5023B GTCAGGATGGCCGAGCGGTCTAA LeuCAG AS-NR-002-1-131
5024A GTTAAGATGGCAGA LeuTAA AS-NR-002-1-132
5026A GTTTCCGTAGTGTAGTGG ValCAC AS-NR-002-1-133
5026/27B GTTTCCGTAGTGTAGTGGTCATC
GTTTCCGTAGTGTAGTGGTTATC
ValAAC
ValCAC
ValAAC
AS-NR-002-1-134
5028/29A TCCCACATGGTCTAGCGG
TCCCATATGGTCTAGCGG
GluTTC
GluTTC
AS-NR-002-1-135
5028/29B TCCCACATGGTCTAGCGGTTAGG
TCCCATATGGTCTAGCGGTTAGG
GluTTC
GluTTC
AS-NR-002-1-136
5032A TCCTCGTTAGTATAGTGG AspGTC AS-NR-002-1-137
5032B TCCTCGTTAGTATAGTGGTGAGT AspGTC AS-NR-002-1-138
TRF21-26 ACCAGAATGGCCGAGTGGTT LeuTAA AS-NR-002-1-139
TRF63 AGCAGAGTGGTGCAGTGG MetCAT AS-NR-002-1-140
TRF23 ACCCTGTGGTCTAGTGG GluTTC AS-NR-002-1-141
TRF205 CCCCTGGTGGTCTAGTGCTTAGGATTT GluCTC AS-NR-002-1-142
TRF208 CCCTGTGGTCTAGTGGTTAG GluTTC AS-NR-002-1-143
TRF223 CGCTCTTTGGTCTAGGGG ProAGG AS-NR-002-1-144
TRF250 CTCGTTAGTATAGTGGT AspGTC AS-NR-002-1-145
TRF272/274 GACCTCGTGGCGCAACGG
GACCTCGTGGCGCAATGG
TrpCCA
TrpCCA
AS-NR-002-1-146
TRF273 GACCTCGTGGCGCAACGGT TrpCCA AS-NR-002-1-147
TRF293/294 GCATGGGTGATTCAGTGGTAGAATTT
GCATGGGTGGTTCAGTGGTAGAATTC
GlyGCC
GlyGCC
AS-NR-002-1-148
TRF305/306
307
GCCCCAGTGGCCTAATGGA
GCCCCAGTGGCCTGATGGA
GCCCCGGTGGCCTAATGGA
ArgCCT
ArgCCT
ArgCCT
AS-NR-002-1-149
TRF308 GCCCGACTACCTCAGTCGG LysCTT AS-NR-002-1-150
TRF312 GCCCGGATGATCCTCAGTGGT SeCTCA AS-NR-002-1-151
TRF316 GCCCTCTTAGCGCAGTGGG MetCAT AS-NR-002-1-152
TRF318 GCCGAAATAGCTCAGTTGG PheGAA AS-NR-002-1-153
TRF320 GCCGTGATCGTATAGTGGTTA HisGTG AS-NR-002-1-154
TRF321 GCCTCCTTAGCGCAG MetCAT AS-NR-002-1-155
TRF322 GCCTCGTTAGCGCAGTAGG MetCAT AS-NR-002-1-156
TRF323/324
326
GCCTGGATAGCTCAGTCGG
GCCTGGATAGCTCAGTTGG
GCCTGGGTAGCTCAGTCGG
LysTTT
LysTTT
LysTTT
AS-NR-002-1-157
TRF337-339 GCGTTGGTGGTATAGTGGT GlyTCC AS-NR-002-1-158
TRF347 GCTTCTGTAGTGTAGTGG ValCAC AS-NR-002-1-159
TRF351 GGATTGGTGGTCCAGTGGTAGAATTC ArgCCT AS-NR-002-1-160
TRF354 GGCCCTATAGCTCAGGGG ThrTGT AS-NR-002-1-161
TRF356/359 GGCCGCGTGGCCTAATGGA
GGCCGTGTGGCCTAATGGA
ArgTCG
ArgTCG
AS-NR-002-1-162
TRF368 GGCTCCGTGGCGCAATGGA ArgTCT AS-NR-002-1-163
TRF366 GGCTCCATAGCTCAGTGGTTAGAGCA ThrTGT AS-NR-002-1-164
TRF365 GGCTCCATAGCTCAGGGGT ThrTGT AS-NR-002-1-165
TRF373 GGCTCTGTGGCGCAATGGA ArgTCT AS-NR-002-1-166
TRF374 GGCTCTGTGGCTTAGTTGGC ThrCGT AS-NR-002-1-167
TRF375 GGCTTCGTGGCTTAGCTGG ThrAGT AS-NR-002-1-168
TRF393 GGGGGCATAGCTCAGTGG CysGCA AS-NR-002-1-169
TRF396 GGGGGTATAGCTCAGCGGT AlaAGC AS-NR-002-1-170
TRF417 GGTAGCGTGGCCGAGTGGTCT LeuTAG AS-NR-002-1-171
TRF457 GTAGTCGTGGCCGAGTGG SerAGA AS-NR-002-1-172
TRF460 GTCACGGTGGCCGAGTGG SerCGA AS-NR-002-1-173
TRF462 GTCAGGATGGCCGAGCGGT LeuCAG AS-NR-002-1-174
TRF463 GTCAGGATGGCCGAGTGG LeuCAA AS-NR-002-1-175
TRF466
468
469
471
472
473
GTCTCTGTGGCACAATCGGT
GTCTCTGTGGCGCAATCGG
GTCTCTGTGGCGCAATCGGT
GTCTCTGTGGCGCCATCGGT
GTCTCTGTGGCGTAGTCGGT
GTCTCTGTGGTGCAATCGGT
AsnGTT
AsnGTT
AsnGTT
AsnGTT
AsnGTT
AsnGTT
AS-NR-002-1-176
TRF490 GTTAAGATGGCAGAG LeuTAA AS-NR-002-1-177
TRF492 GTTAAGATGGCAGAGCCTGGT LeuTAA AS-NR-002-1-178
TRF493 GTTAAGATGGCATAGCCC LeuTAA AS-NR-002-1-179
TRF511 GTTTCCGTAGTGTAG ValCAC AS-NR-002-1-180
TRF524 TAGGATGTGGTGTGACAG GlnTTG AS-NR-002-1-181
TRF533/534 TCCCACATGGTCTAGCGGT
TCCCATATGGTCTAGCGGT
GluTTC
GluTTC
AS-NR-002-1-182
TRF537 TCCCCTTGGTCTAGTGGTTAGGATTC GluCTC AS-NR-002-1-183
TRF546/547 TCCTCGTTAGTATAGTGGT
TCCTCATCAGTATAGTGGT
AspGTC
AspGTC
AS-NR-002-1-184
TRF550/551 TCCTTGGTGGTCTAGTGGCTAG
TCCTTGATGTCTAGTGGTTAG
GluTTC
GluCTC
AS-NR-002-1-185

 

House Keeping Gene Catalog Number
RNU6-2 AS-NR-002-1-186
SNORD43 AS-NR-002-1-187
SNORD95 AS-NR-002-1-188