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NuRNA™ Human Small RNA Biogenesis Proteins PCR Array AS-NM-002-1 384-well (2*192) / plate $435.00
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NuRNA™ Human Small RNA Biogenesis Proteins PCR Array (Roche Light Cycler 480) AS-NM-002-1-R 384-well (2*192) / plate $435.00
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Small RNAs are noncoding RNA molecules < 200 nt in length. Eukaryotic small RNAs are classified into biotypes based on their molecular characteristics and fundamental biological functions: microRNAs (miRNA), PIWI-interacting RNAs (piRNA), short interfering RNAs (siRNA), small nucleolar RNAs (snoRNA), small nuclear RNAs (snRNA), transfer RNAs (tRNA), and tRNA-related fragments and halves (tRF) (Figure 1). Some types of small RNAs perform fundamental functions, such as tRNA in protein translation and snoRNA in rRNA modification. Some others are regarded as small regulatory RNAs which regulate gene expression and modulate biological activities by multiple mechanisms, such as mRNA stability and translation control, or targeting epigenetic modifications to specific genomic regions [1-3]. Their stability, enrichment abundance in biofluids have been utilized for biomarker applications.

Small RNA biogenesis pathways are the underlying processes that produce these small RNAs and are regulated in response to cellular conditions. Disorders of the pathways are often associated with diseases. For example, the expression levels of DROSHA and DICER1, the key protein factors in miRNA biogenesis, change along with the stages of tumor progression, which have been investigated as a potential biomarker for neuroblastoma prognosis [4]. Profiling small RNAs biogenesis pathways facilitates the study of the small RNAs, the biological impacts and the associated diseases.

PCR Arrays are the most reliable tools for analyzing the expression of a focused panel of genes. For rapid and convenient expression profiling of protein and enzymatic components involved in small RNA biogenesis, Arraystar has developed the first commercial small RNA biogenesis PCR panel. Arraystar NuRNA™ Human Small RNA Biogenesis Proteins PCR Array profiles the expression of 185 protein coding mRNA genes categorized in the small RNA biogenesis gene ontological (GO) pathways. The Spike-in, Positive PCR, Genomic DNA and normalization reference controls are included to ensure the upmost data quality.

1

Figure 1. Biogenesis and functions of small RNA biotypes.

References

[1] Kim VN. Small RNAs: classification, biogenesis, and function. Molecules and cells 2005;19:1-15.
[2] Esteller M. Non-coding RNAs in human disease. Nature reviews Genetics 2011;12:861-74.
[3] Matera AG, Terns RM, Terns MP. Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs. Nature reviews Molecular cell biology 2007;8:209-20.
[4] Lin RJ, Lin YC, Chen J, Kuo HH, Chen YY, Diccianni MB, et al. microRNA signature and expression of Dicer and Drosha can predict prognosis and delineate risk groups in neuroblastoma. Cancer research 2010;70:7841-50.

Human Small RNA Biogenesis-related Proteins (185 transcripts)

miRNA biogenesis (57):

ADAR; AGO1; AGO2; AGO3; AGO4; BCDIN3D; CNOT1; CNOT10; CNOT11; CNOT2; CNOT3; CNOT4; CNOT6; CNOT6L; CNOT7; CNOT8; DGCR8; DICER1; DIS3L2; DROSHA; EIF6; ESR1; ETS1; FOSL1; HNRNPA2B1; HRAS; KHSRP; LIN28B; METTL14; METTL3; MOV10; MRPL44; NCOR1; NCOR2; NFKB1; PNPT1; PPP3CA; PRKRA; PTBP1; RAN; RBM4; RELA; RPL7A; RQCD1; SMAD1; SMAD2; SMAD3; SNIP1; SRRT; SUPV3L1; TARBP2; TNRC6A; TNRC6B; TNRC6C; XPO5; ZC3H12A; ZCCHC11

piRNA biogenesis (17):

ASZ1; DDX4; EXD1; FKBP6; HENMT1; MAEL; MOV10L1; PIWIL1; PIWIL2; PIWIL4; PLD6; TDRD1; TDRD12; TDRD6; TDRD7; TDRD9; TDRKH

siRNA biogenesis (24):

ADAR; AGO1; AGO2; AGO3; AGO4; CELF1; CLP1; DICER1; DROSHA; MBD2; MBD3; MECP2; MRPL44; NRDE2; PRKRA; TARBP2; TERT; TLR7; TLR9; TSEN15; TSEN2; TSEN34; TSEN54; XPO5

snoRNA biogenesis (24):

AQR; DKC1; EXOSC2; EXOSC3; EXOSC4; EXOSC5; EXOSC6; FBL; FBLL1; GAR1; NAF1; NOP10; NOP56; NOP58; NUDT16; PIH1D1; PRPF31; RNF113A; RNF113B; RPAP3; RUVBL1; RUVBL2; SNU13; ZNHIT6

snRNA biogenesis (41):

CPSF3L; DKC1; EXOSC2; EXOSC3; EXOSC4; EXOSC5; EXOSC6; EXOSC7; EXOSC8; EXOSC9; INTS1; INTS10; INTS12; INTS2; INTS3; INTS4; INTS5; INTS6; INTS7; INTS8; INTS9; KPNB1; MEPCE; NCBP1; NCBP2; NHP2; NOP10; PHAX; RPAP2; SMN1; SNAPC1; SNAPC2; SNAPC3; SNAPC4; SNAPC5; SNUPN; TGS1; TUT1; USB1; XPO1; ZPR1

tRNA & tRF biogenesis (47):

AGO1; AGO2; AGO3; AGO4; ANG; DGCR8; DICER1; EIF2AK4; ELAC1; ELAC2; HSD17B10; KIAA0391; NUP107; NUP153; NUP155; NUP160; NUP188; NUP210; NUP214; NUP35; NUP37; NUP43; NUP54; NUP62; NUP93; NUP98; NUPL2; PIWIL4; POM121C; POP1; POP4; POP5; POP7; PTCD1; RAE1; RANBP2; RPP14; RPP21; RPP25; RPP30; RPP38; RPP40; TPR; TRMT10C; TRNT1; XPOT; Ybx1

• Best content — Comprehensive selection of small RNAs biogenesis-related proteins categorized in the GO pathways. 
• High performance — All primer pairs and assays are highly optimized by design and rigorously validated.
• Convenient — Easy-to-use, ready-to-run, standard qPCR plate format for direct sample application. No sample pre-amplification is needed.
• Complete with Arraystar’s broad selection of small RNA analysis technologies, from small RNA sequencing, qPCR panel, to modification detection by LC-MS/MS.

 

AS-NM-002-overview-protocol

Compatible qPCR Instruments:
ABI ViiA™ 7
ABI 7500 & ABI 7500 FAST
ABI 7900HT
ABI QuantStudio™ 6 Flex Real-Time PCR system
ABI QuantStudio™ 7 Flex Real-Time PCR system
ABI QuantStudio™ 12K Flex Real-Time PCR System
Bio-Rad CFX384
Bio-Rad iCycler & iQ Real-Time PCR Systems
Eppendorf Realplex
QIAGEN Rotor Gene Q 100
Roche Light Cycler 480
Stratagene Mx3000
Roche Light Cycler 480