• Description
  • tRNA Repertoire PCR Array Service
  • tRF&tiRNA PCR Array Service
  • Functional LncRNA PCR Array Service
  • snoRNA PCR Array Services
Service NameSizePrice
nrStar™ Human tRNA Repertoire PCR Array Service 384-well (4*96)plate
nrStar™ Human tRF&tiRNA PCR Array Service 384-well(2*192) / plate
nrStar™ Human Functional LncRNA PCR Array Service 384-well/ plate
nrStar™ Human snoRNA PCR Array Service 384 well / plate

Non-coding RNAs (ncRNA) comprise large proportions of RNAs in the cells. ncRNA classes, such as tRNA, tRF & tiRNA, snoRNA, and lncRNA, have diverse and fundamental biological functions, often with additional non-canonical, regulatory functions or association with human diseases. Arraystar has pioneered the first series of PCR panels dedicated for the expression profiling studies of these ncRNA classes. In addition to these panels as products, Arraystar also launches full ncRNA PCR Array services from sample to data. The services muster the expertise, performance and power of specially optimized rtStar™ cDNA synthesis dedicated for the unique ncRNA properties and modifications. The services deliver a wealth of data and rich analyses to project your research into the new front of ncRNA science and applications.

1.6

Figure 1. The fold changes in expression levels of lncRNAs profiled by nrStar™ Functional LncRNA PCR Array. Left: Top 20 up-regulated lncRNAs. Right: Top 20 down-regulated lncRNAs.

nrStar™ Human tRNA Repertoire PCR Array profiles nuclear and mitochondrial tRNAs of all anticodons to analyze fundamentally important codon preferences, protein translation efficiency, accuracy, cellular dynamics, and viral infection tropism. 
rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit (Arraystar, Cat# AS-FS-004) contains a powerful RNA demethylase that efficiently removes the tRNA methylations and greatly improves the cDNA synthesis

Specifications and workflow

RNA sample source

Total RNA extracted by standard TRIZol methods

Sample requirements

1.5 µg total RNA amount, with OD260/280 ratio ~ 2 and OD260/230 ratio ~ 2

qPCR references and controls

Small RNA References: RNU6-2, SNORD43, and SNORD95;

RNA Spike-in Control;

Genomic DNA Control;

Positive PCR Control;

Blank Control

cDNA conversion

Reverse transcription with random primers after demethylation treatment

Reporter

SYBR Green

Assay format

384-well plate (4*96)

nrStar™ Human tRF&tiRNA PCR Array profiles tRFs and tiRNAs to study the functions of tRNA derived fragments and tRNA halves as a small ncRNA class and to discover the biomarker potentials for their abundance even higher than miRNAs in biofluids.
rtStar™ cDNA synthesis kit (Arraystar, Cat# AS-FS-003) discriminates tRFs & tiRNAs from their tRNA precursors and other small RNAs, producing reliable and accurate detection and quantification of tRF&tiRNA expression.

Specifications and workflow

RNA sample source

Total RNA extracted by standard TRIZol methods

Sample requirements

1.5 µg total RNA, with OD260/280 ratio ~ 2 and OD260/230 ratio ~ 2

qPCR references and controls

Small RNA Reference: RNU6-2, SNORD43, and SNORD95;

RNA Spike-in Control;

Genomic DNA Control;

Positive PCR Control;

Blank Control

cDNA conversion

Activated adaptor ligation for universal RT primed reverse transcription

Reporter

SYBR Green

Assay format

384-well plate (2*192)

 

nrStar™ Human Functional LncRNA PCR Array profiles 372 Gold lncRNAs well characterized for the key roles in regulatory functions in biology and disease, and the expressional specificities as biomarkers. 
rtStar™ cDNA synthesis kit (AS-FS-001) guarantees efficient and robust conversion of lncRNAs into cDNAs, for both poly(A ) and poly(A-), intact and degraded lncRNA samples, extracted from cells or biofluids.

Specifications and workflow

RNA sample source

Total RNA extracted by standard TRIZol methods

Sample requirements

1.5 µg total RNA with OD260/280 ratio ~ 2 and OD260/230 ratio ~ 2

qPCR references and controls

Human housekeeping genes: ACTB, B2M, Gusb, Hsp90ab1, GAPDH and 18S rRNA;

RNA Spike-in Control;

Genomic DNA Control;

Positive PCR Control;

Blank Control

cDNA conversion

Reverse transcription with random primers and oligo (dT)

Reporter

SYBR Green

Assay format

384-well plate

nrStar™ Human snoRNA PCR Array profiles snoRNAs, target snRNAs and snoRNP complex members, whose dysregulation is prominent in cancers and neurodegenerative diseases, and stable abundance desired as a biomarker. 
rtStar™ cDNA synthesis kit (AS-FS-001) guarantees efficient and robust conversion of snoRNAs into cDNAs, for in intact or degraded RNA samples, extracted from cells or biofluids.

Specifications and workflow

RNA sample source

Total RNA extracted by standard TRIZol methods

Sample requirements

1.5 µg total RNA with OD260/280 ratio ~ 2 and OD260/230 ratio ~ 2

qPCR references and controls

Human housekeeping genes: ACTB, B2M, Gusb, Hsp90ab1, GAPDH, 5S rRNA, 28S rRNA and 18S rRNA;

RNA Spike-in Control;

Genomic DNA Control;

Positive PCR Control;

Blank Control

cDNA conversion

Reverse transcription with random primers

Reporter

SYBR Green

Assay format

384-well plate