Arraystar nrStar™ PCR Arrays have the most comprehensive, and up-to-date best content on the market. nrStar™ snoRNA PCR Arrays profile 359 human snoRNAs/370 mouse snoRNAs, which are derived from two authoritative snoRNA database (SnoPY and snoRNA-LBME-db). The panel covers all the prevalently detected snoRNAs as a powerful tool for snoRNA profiling and functional research.
Benefits
• Excellent coverages – For all the PCR-distinguishable snoRNAs, snoRNA targets, and snoRNP members in one Panel.
• Unmatched sensitivity - As little as 50 ng total RNA. No pre-amplification necessary.
• Proven performance - All snoRNAs have been fully experimentally validated with cell/tissue/serum/plasma samples.
• Fast and easy - Ready-to-use format for direct sample application. Just mix the converted cDNA and SYBR® Green master mix to the plates and run the real-time PCR. The entire process can be completed under 4 hours.
• Biomarker discovery - The coverage, sensitivity, accuracy, and throughput are excellent for biomarker screening and validation work.
We recommend using our rtStar™ First-Strand cDNA Synthesis Kit (AS-FS-001) along with Arraystar SYBR® Green Real-time qPCR Master Mix when running your nrStar™ snoRNA PCR Arrays for optimal performance.
| Product Name | Catalog No | Size | Price |
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nrStar™ Human snoRNA PCR Array
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AS-NR-003-1 |
384 well / plate |
$403.00
Add To Cart
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nrStar™ Human snoRNA PCR Array (Roche Light Cycler 480)
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AS-NR-003-1-R |
384 well / plate |
$403.00
Add To Cart
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nrStar™ Mouse snoRNA PCR Array
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AS-NR-003M-1 |
384 well / plate |
$403.00
Add To Cart
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nrStar™ Mouse snoRNA PCR Array (Roche Light Cycler 480)
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AS-NR-003M-1-R |
384 well / plate |
$403.00
Add To Cart
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Small nucleolar RNAs (snoRNAs) are intermediate-length small noncoding RNAs (sncRNAs), ranging from 60 to 300 nt. They are a vital component of small nucleolar ribonucleoprotein (snoRNPs) [1]. In vertebrates, the snoRNA genes are frequently embedded within the introns of protein coding genes and post-transcriptionally processed [2]. snoRNAs are involved in rRNA processing and regulation of splicing, translation, and oxidative stress [3]. Within snoRNPs, snoRNAs complement with rRNA sequences and guide the modification of 2’ O-methylation or pseudouridylation. RNPs that catalyze 2’ O-methylation are called C/D box RNPs, while those that catalyze pseudouridylation are termed H/ACA box RNPs [1]. Besides these snoRNAs, small Cajal body-specific RNAs (scaRNA) in the primary structures of small Cajal body in the nucleus frequently contain similar box C/D and box H/ACA domains. They are structurally indistinguishable from canonical snoRNAs [4]. Also, some snoRNAs can be processed by Dicer and give rise to miRNA-like RNAs, capable of binding to AGO and complementing with mRNA and thus regulating the translation [5].
Dysregulated expression of snoRNAs in cancer and their roles in metastasis are well documented. SnoRNAs can act either as tumor promoters or suppressors [6-7]. For example, SNORD50A/B act as tumor suppressors which directly bind and inhibit K-Ras and are recurrently deleted in human cancer [8]; SNORD44 (RNU44) and SNORD43 (RNU43) are associated with poor prognosis of breast cancer [9]; and SNORA80E (SNORA42) can act as oncogene in lung cancer and its siRNA knock down has shown anti-cancer effects [10]. Interestingly, C/D box snoRNAs are globally increased in cancer [11]. snoRNA expression is also dysregulated in neurodegenerative disorders. snoRNAs are relatively stable and have been detected in blood plasma, sputum, and urine samples, presenting a promising biomarkers for diagnostics and targets for treatment. Studying snoRNAs has become a vibrant field of research.
nrStar™ Human/Mouse snoRNA PCR Array covers all the human/mouse snoRNAs distinguishable by PCR. The array is a powerful tool to conveniently and quickly analyze the expression of snoRNAs from sample sources such as cancer tissue, cultured cells and biofluids such as serum or plasma. The coverage, sensitivity and accuracy are excellent for disease biomarker screening and validation.
To ensure high data quality, the panel includes multiple reference sets to better quantify and normalize the qPCR data: 3 reference sets for human snoRNAs & 2 reference sets for human SNP complex member mRNAs or 8 reference sets for mouse snoRNAs. Potential genomic DNA contamination is monitored by using the genomic DNA control (GDC).
For cDNA preparation, we highly recommend rtStar™ First-Strand cDNA Synthesis Kit (AS-FS-001), of which reagent formulations and protocols have been rigorously tested and optimized for Arraystar PCR Array system.
References
1. Esteller M. (2011) "Non-coding RNAs in human disease." Nat. Rev. Genet. 12(12):861-74 [PMID: 22094949]2. Richard P. and T. Kiss (2006) "Integrating snoRNP assembly with mRNA biogenesis." EMBO Rep. 7(6):590-2 [PMID: 16741502]
3. Williams G.T. and F. Farzaneh (2012) "Are snoRNAs and snoRNA host genes new players in cancer?" Nat. Rev. Cancer 12(2):84-8 [PMID: 22257949]
4. Darzacq X. et al. (2002) "Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs." EMBO J. 21(11):2746-56 [PMID: 12032087]
5. Ender C. et al. (2008) "A human snoRNA with microRNA-like functions." Mol. Cell 32(4):519-28 [PMID: 19026782]
6. Nallar S.C. and D.V. Kalvakolanu (2013) "Regulation of snoRNAs in cancer: close encounters with inter*****." J. Inter***** Cytokine Res. 33(4):189-98 [PMID: 23570385]
7. Mannoor K. et al. (2012) "Small nucleolar RNAs in cancer." Biochim. Biophys. Acta 1826(1):121-8 [PMID: 22498252]
8. Siprashvili Z. et al. (2016) "The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer." Nat. Genet. 48(1):53-8 [PMID: 26595770]
9. Gee H.E. et al. (2011) "The small-nucleolar RNAs commonly used for microRNA normalisation correlate with tumour pathology and prognosis." Br. J. Cancer 104(7):1168-77 [PMID: 21407217]
10. Mei Y.P. et al. (2012) "Small nucleolar RNA 42 acts as an oncogene in lung tumorigenesis." Oncogene 31(22):2794-804 [PMID: 21986946]
11. Su H. et al. (2014) "Elevated snoRNA biogenesis is essential in breast cancer." Oncogene 33(11):1348-58 [PMID: 23542174]
SnoPY :http://snoopy.med.miyazaki-u.ac.jp/
snoRNA-LBME-db:http://www-snorna.biotoul.fr/
Human snoRNA database
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C/D BOX(213)
SNORD4A; SNORD5; SNORD6; SNORD7; SNORD8; SNORD9; SNORD10; SNORD119; SNORD121A; SNORD121B; SNORD123; SNORD124; SNORD126; SNORD127; SNORD1A; SNORD1B; SNORD1C; SNORD2; SNORD101; SNORD102; SNORD103; SNORD103B; SNORD104; SNORD105; SNORD105B; SNORD12C; SNORD13; SNORD14A; SNORD14B; SNORD15A; SNORD15B; SNORD16; SNORD18A; SNORD18B; SNORD18C; SNORD20; SNORD21; SNORD22; SNORD24; SNORD25; SNORD26; SNORD27; SNORD28; SNORD3; SNORD30; SNORD31; SNORD32A; SNORD32B; SNORD33; SNORD34; SNORD35A; SNORD35B; SNORD36A; SNORD36B; SNORD36C; SNORD37; SNORD38A; SNORD38B; SNORD41; SNORD42A; SNORD42B; SNORD43; SNORD4B; U97; SNORD96B; SNORD96A; SNORD95; SNORD94; SNORD86; SNORD84; SNORD83B; SNORD83A; SNORD117; SNORD82; SNORD81; SNORD80; SNORD118; SNORD78; SNORD77; SNORD76; SNORD75; SNORD73a; SNORD63; SNORD62B; SNORD61; SNORD60; SNORD59B; SNORD59A; SNORD58B; SNORD58C; SNORD57; SNORD56; SNORD55; SNORD54; SNORD53; SNORD52; SNORD51; SNORD50; SNORD50B; SNORD48; SNORD47; SNORD46; SNORD45A; SNORD45B; SNORD45C; SNORD44; SNORD12; SNORD12B; SNORD112; SNORD113-1; SNORD114-1; SNORD17; SNORD19; SNORD19B; SNORD23; SNORD65; SNORD66; SNORD67; SNORD88A/B/C; SNORD68; SNORD69; SNORD70; SNORD71; SNORD72; SNORD85; SNORD87; SNORD90; SNORD91A; SNORD91B; SNORD92; SNORD93; SNORD98; SNORD99; SNORD100; SNORD109A/B; SNORD115-1; SNORD110; SNORD111; SNORD111B; SNORD116-1; SNORD11; SNORD11B; SNORD12; SNORD12B; SNORD113-2; SNORD113-4;SNORD113-5; SNORD113-6; SNORD113-7; SNORD113-8; SNORD113-9/ SNORD113-3; SNORD114-10/18; SNORD114-11; SNORD114-12/14; SNORD114-13; SNORD114-15/3/5/21/23/25/26; SNORD114-17/4; SNORD114-19; SNORD114-2; SNORD114-20/21/22/28; SNORD114-24; SNORD114-29/30; SNORD114-31; SNORD114-6/9; SNORD114-7; SNORD115-11; SNORD115-12; SNORD115-17/18/23; SNORD115-27/29/30; SNORD115-28; SNORD115-36; SNORD115-37; SNORD115-48; SNORD116-11; SNORD116-12/16/17/18/21/22/24; SNORD116-13/14/15/20; SNORD116-19; SNORD116-23; SNORD116-25/26; SNORD116-27/29/30; SNORD116-28; SNORD116-5; SNORD116-6; SNORD116-9; SNORD79; SNORD58A; SNORD49A; SNORD49B; SNORD45BL2; SNORD42BL1; SNORD3@L39; SNORD3@L19; SNORD118L14; SNORD3L3; SNORD118L11; SNORD68L1; SNORD118L9; SNORD3@L37; SNORD74L5; SNORD45BL1; SNORD65L2; SNORD23; SNORD38BL3; SNORD44L1; SNORD74L4; SNORD45BL3; SNORD56L7; SNORD77L3; SNORD41L1; SNORA25L14; SNORD75L2; SNORD114-15L1; U3.41-201; SNORD53L1; SNORD55L1; SNORD62BL1; SNORD51L1;
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H/ACA BOX(122)
SNORA10; SNORA13; SNORA14A; SNORA14B; SNORA15; SNORA16; SNORA17; SNORA18; SNORA19; SNORA21; SNORA22; SNORA23; SNORA24; SNORA25; SNORA28; SNORA3; SNORA2B; SNORA45; SNORA30; SNORA31; SNORA33; SNORA34; SNORA36; SNORA36B; SNORA37; SNORA38; SNORA39; SNORA4; SNORA41; SNORA42; SNORA43; SNORA44; SNORA46; SNORA48; SNORA49; SNORA5A; SNORA50; SNORA51; SNORA52; SNORA53; SNORA54; SNORA55; SNORA56; SNORA58; SNORA59; SNORA5B; SNORA5C; SNORA6; SNORA60; SNORA61; SNORA77; SNORA80; SNORA80B; SNORA7A; SNORA8; SNORA9; SNORA62; SNORA63; SNORA47; SNORA35; SNORA26; SNORA11B; SNORA11D; SNORA11E; SNORA36C; SNORA38B; SNORA84; SNORA11; SNORA12; SNORA73A; SNORA73B; SNORA74A; SNORA74B; SNORA64; SNORA65; SNORA66; SNORA67; SNORA70; SNORA70B; SNORA70C; SNORA70D; SNORA70E; SNORA70F; SNORA71A; SNORA71B; SNORA72; SNORA99; SNORA71D; SNORA20; SNORA27; SNORA29; SNORA32; SNORA40; SNORA78; HBI-61; SNORA11C; SNORA68; SNORA69; SNORA16B; SNORA1; SNORA72L7; SNORA64L2; SNORA18L1; SNORA62L2; AL137790.4; SNORA11DL1; SNORA51L11; SNORA10L1; SNORA12L2; SNORA11EL1; SNORA70BL6; SNORA20L1; SNORA63L9; SNORA18L2; SNORA20L4; SNORA11BL2; SNORA67L1; SNORA32L2; SNORA2AL1; SNORA43L2; SNORA12L1;SNORA62L4
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Cajal body-specific scaRNAs(24)
SCARNA18; HTR; HBII-382; SCARNA13; SCARNA8; SCARNA17; SCARNA7; SCARNA12; SCARNA6; SCARNA5; SCARNA10; SCARNA14; mgU2-25/61; SCARNA9; mgU12-22/U4-8; HBI-100; ACA68; ACA66; SCARNA11; SCARNA16; SCARNA1; SCARNA4; SCARNA23; SCARNA22;
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Mouse snoRNA database
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C/D BOX(134)
300055_SNORD116; 300078_SNORD49; 300079_SNORD49; 300080_SNORD65; 300108_SNORD38; 300110_SNORD50; 300116_SNORD115; 300118_SNORD115; 300144_SNORD115; 300189_SNORD113; 300194_SNORD93; 300222_SNORD115; 300273_SNORD113; 300274_SNORD113; 300275_SNORD113; 300277_SNORD113; 300278_SNORD113; 300308_SNORD58; 300357_SNORD60; 300375_SNORD12; 300441_SNORD115; 300489_SNORD86; 300524_SNORD86; 300528_SNORD113; 300529_SNORD113; 300530_SNORD113; 300531_SNORD113; 300532_SNORD113; 300537_SNORD64; 300543_SNORD89; 300559_SNORD30; 300561_SNORD113; 300562_SNORD14; 300613_SNORD99; 300615_SNORD52; 300616_SNORD48; 300617_SNORD52; 300638_SNORD78; 300639_SNORD79; 300641_SNORD47; 300642_SNORD81; 300645_SNORD115; 300647_SNORD115; 300662_SNORD115; 300674_SNORD116; 300677_SNORD70; 300706_SNORD81; 300709_SNORD25; 300710_SNORD26; 300711_SNORD27; 300712_SNORD28; 300713_SNORD29; 300714_SNORD30; 300715_SNORD31; 300716_SNORD22; 300721_SNORD87; 300732_SNORD63; 300734_SNORD58; 300739_SNORD94; 300746_SNORD23;300751_SNORD88; 300752_SNORD35; 300753_SNORD35; 300754_SNORD34; 300756_SNORD32; 300759_SNORD15; 300760_SNORD15; 300771_SNORD21; 300778_SNORD102; 300782_SNORD70; 300784_SNORD51; 300789_SNORD20; 300790_SNORD82; 300795_SNORD74; 300796_SNORD75; 300797_SNORD24; 300808_SNORD41; 300812_SNORD71; 300813_SNORD111; 300814_SNORD111; 300818_SNORD69; 300819_SNORD19; 300820_SNORD19; 300832_SNORD61; 300843_SNORD100; 300844_SNORD101; 300845_SNORD98; 300846_SNORD37; 300851_SNORD59; 300852_SNORD72; 300858_SNORD43; 300873_SNORD66; 300886_SNORD105; 300887_SNORD14; 300888_SNORD14; 300889_SNORD14; 300890_SNORD18; 300891_SNORD16; 300892_SNORD18; 300893_SNORD16; 300894_SNORD18; 300903_SNORD73; 300904_SNORD73; 300907_SNORD62; 300909_SNORD45; 300910_SNORD45; 300916_SNORD83; 300917_SNORD83; 300918_SNORD92; 300919_SNORD53; 300920_SNORD53; 300930_SNORD95; 300931_SNORD96; 300936_SNORD91; 300937_SNORD42; 300939_SNORD42; 300954_SNORD38; 300955_SNORD38; 300956_SNORD46; 300957_SNORD55; 300961_SNORD85; 300962_SNORD103; 300966_SNORD24; 300967_SNORD36; 300968_SNORD36; 300974_SNORD90; 300976_SNORD67; 300980_SNORD110; 300982_SNORD56; 300983_SNORD57; 300985_SNORD17; 300988_SNORD12; 300989_SNORD12; 300990_SNORD12
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H/ACA BOX(221)
300000_SNORA17; 300001_SNORA65; 300003_SNORA17; 300004_SNORA73; 300010_SNORA17; 300011_SNORA17; 300013_SNORA75; 300016_SNORA51; 300019_SNORA25; 300020_SNORA43; 300021_SNORA17; 300023_SNORA2; 300025_SNORA44; 300031_SNORA17; 300033_SNORA76; 300037_SNORA17; 300038_SNORA17; 300040_SNORA17; 300044_SNORA2; 300045_SNORA44; 300049_SNORA70; 300074_SNORA63; 300075_SNORA25; 300089_SNORA58; 300092_SNORA30; 300097_SNORA8; 300098_SNORA1; 300100_SNORA32; 300102_SNORA17; 300104_SNORA73; 300111_SNORA17; 300112_SNORA32; 300113_SNORA17; 300115_SNORA70; 300167_SNORA33; 300175_SNORA73; 300177_SNORA17; 300195_SNORA17; 300196_SNORA17; 300265_SNORA17; 300266_SNORA70; 300271_SNORA70; 300279_SNORA71; 300284_SNORA17; 300285_SNORA17; 300288_SNORA70; 300289_SNORA17; 300295_SNORA71; 300304_SNORA9; 300311_SNORA61; 300312_SNORA17; 300313_SNORA17; 300315_SNORA67; 300319_SNORA32; 300320_SNORA70; 300322_SNORA38; 300327_SNORA17; 300330_SNORA28; 300343_SNORA71; 300354_SNORA46; 300358_SNORA43; 300367_SNORA65; 300368_SNORA17; 300385_SNORA17; 300391_SNORA31; 300393_SNORA17; 300398_SNORA71; 300400_SNORA46; 300401_SNORA67; 300406_SNORA17; 300410_SNORA57; 300411_SNORA67; 300413_SNORA68; 300414_SNORA17; 300417_SNORA71; 300418_SNORA71; 300421_SNORA4; 300428_SNORA55; 300430_SNORA66; 300432_SNORA17; 300434_SNORA70; 300447_SNORA17; 300451_SNORA13; 300456_SNORA2; 300458_SNORA17; 300461_SNORA2; 300464_SNORA17; 300467_SNORA42; 300468_SNORA3; 300477_SNORA24; 300479_SNORA25; 300480_SNORA17; 300486_SNORA51; 300490_SNORA55; 300491_SNORA17;300496_SNORA17; 300498_SNORA44; 300500_SNORA24; 300501_SNORA44; 300512_SNORA17; 300515_SNORA17; 300526_SNORA79; 300533_SNORA71; 300534_SNORA61; 300535_SNORA7; 300548_SNORA17; 300550_SNORA74; 300555_SNORA63; 300564_SNORA73; 300586_SNORA73; 300590_SNORA17; 300591_SNORA17; 300595_SNORA70; 300608_SNORA17; 300610_SNORA16; 300622_SNORA70; 300623_SNORA2; 300624_SNORA38; 300626_SNORA7; 300629_SNORA55; 300632_SNORA17; 300635_SNORA73; 300637_SNORA17; 300643_SNORA63; 300668_SNORA42; 300670_SNORA51; 300673_SNORA70; 300675_SNORA20; 300693_SNORA24; 300694_SNORA62; 300697_SNORA17; 300703_SNORA71; 300704_SNORA26; 300707_SNORA61; 300718_SNORA17; 300720_SNORA38; 300724_SNORA44; 300730_SNORA17; 300731_SNORA70; 300738_SNORA5; 300740_SNORA73; 300741_SNORA36; 300742_SNORA63; 300743_SNORA7; 300745_SNORA70; 300747_SNORA40; 300757_SNORA70; 300758_SNORA32; 300763_SNORA13; 300764_SNORA23; 300765_SNORA24; 300766_SNORA17; 300768_SNORA52; 300769_SNORA54; 300775_SNORA49; 300777_SNORA22; 300779_SNORA27; 300781_SNORA33; 300785_SNORA41; 300787_SNORA26; 300788_SNORA75; 300793_SNORA61; 300798_SNORA17; 300800_SNORA19;300801_SNORA42; 300802_SNORA12; 300803_SNORA32; 300805_SNORA3; 300807_SNORA68; 300809_SNORA46; 300810_SNORA50; 300817_SNORA14; 300821_SNORA74; 300823_SNORA26; 300824_SNORA36; 300825_SNORA31; 300826_SNORA5; 300828_SNORA36; 300829_SNORA69; 300831_SNORA17; 300834_SNORA72; 300835_SNORA35; 300836_SNORA11; 300839_SNORA70; 300847_SNORA53; 300848_SNORA79; 300853_SNORA17; 300854_SNORA72; 300856_SNORA11; 300859_SNORA2; 300861_SNORA73; 300862_SNORA55; 300863_SNORA24; 300870_SNORA17; 300872_SNORA77; 300874_SNORA63; 300875_SNORA63; 300877_SNORA17; 300882_SNORA2; 300883_SNORA24; 300897_SNORA61; 300898_SNORA62; 300899_SNORA73; 300900_SNORA62; 300901_SNORA69; 300905_SNORA58; 300908_SNORA5; 300912_SNORA29; 300913_SNORA20; 300914_SNORA64; 300923_SNORA21; 300926_SNORA28; 300927_SNORA5; 300929_SNORA24; 300933_SNORA29; 300949_SNORA17; 300958_SNORA18; 300965_SNORA32; 300977_SNORA11; 300979_SNORA25; 300986_SNORA71
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Other snoRNAs(15)
300032_SNORND104; 300099_snoZ40;300317_snoZ30; 300640_snosnR60_Z15; 300755_snoZ195; 300792_snoU6-53; 300816_snoMBII-202; 300822_snoU6-53; 300857_snoU83B; 300871_snoU2-30; 300884_snoU2-30; 300938_snoZ17; 300947_snR38-2; 300948_snR38-3
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Fig 1. Workflow overview of Human snoRNA PCR Array experiment.
Compatible qPCR Instruments Equipped with a 384-well Format Block:
ABI ViiA™ 7
ABI 7500 & ABI 7500 FAST
ABI 7900HT
ABI QuantStudio™ 5 Real-Time PCR system
ABI QuantStudio™ 6 Flex Real-Time PCR system
ABI QuantStudio™ 7 Flex Real-Time PCR system
ABI QuantStudio™ 12K Flex Real-Time PCR System
Bio-Rad CFX384
Bio-Rad iCycler & iQ Real-Time PCR Systems
Eppendorf Realplex
Stratagene Mx3000
Roche LightCycler 480
Mechanism of Dcp2/RNCR3/Dkc1/Snora62 axis regulating neuronal apoptosis in chronic cerebral ischemia. Yang J, et al. Cell Biology and Toxicology, 2023
Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. Han C, et al. Cell Reports, 2022
Glioma glycolipid metabolism: MSI2–SNORD12B–FIP1L1–ZBTB4 feedback loop as a potential treatment target. Dong W, et al. Clinical and Translational Medicine, 2021
Protein-RNA interactome analysis reveals wide association of KSHV ORF57 with host non-coding RNAs and polysomes. Han C, et al. Cell Reports, 2021
DKC1 Overexpression Induces a More Aggressive Cellular Behavior and Increases Intrinsic Ribosomal Activity in Immortalized Mammary Gland Cells. Guerrieri A N, et al. Cancers, 2020