microRNA Sequencing Service

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Arraystar offers a complete microRNA-seq service from RNA preparation and QC, library construction, sequencing, to comprehensive data analyses.

Benefits

Technological Performance

• Highly optimized procedures to maximize the mappable reads and deliver precise quantification

• Identification of isomiRs, and distinction of mature miRNAs from pre-microRNAs

• No cross-hybridization ambiguity issues

• Sensitive detection for low abundance miRNAs at single-copy per cell level

• Cost-effective highly optimized full service from Sample to Data

Coverage and Annotation

• Applicable to any species, not dependent on prior miRNAs reference

• Always annotated with the latest miRBase

Advanced Analyses 

• Novel miRNA discovery and secondary structure prediction 

• Target gene prediction and network 

• Gene ontology/pathway analysis of miRNA Target Genes 


Promo: Min 6 samples, valid 07/01/23 - 09/30/23.

Service NamePrice
microRNA Sequencing Service

Novel miRNA discovery and secondary structure prediction (Fig. 1)

miRNA_seq-1

 

Target gene prediction and network (Fig. 2)

miRNA_seq-2

 

Gene ontology/pathway analysis of miRNA Target Genes (Fig. 3)

miRNA_seq-3

Your samples
Purified total RNA, or frozen tissues, or cell pellets

Please refer to Sample Submission for details in how to get your project started.

Arraystar microRNA seq

1. RNA isolation (Optional)
2. RNA QC
3. Sequencing library construction and library QC
4. Sequencing by illumina platform
5. Raw read data processing
6. Data analyses and report
7. Project data delivery

1a,25-Dihydroxyvitamin D3 Regulates microRNA Packaging in Extracellular Matrix Vesicles and Their Release in the Matrix. Asmussen N C,et al. Calcified Tissue International, 2023

Changes in subcutaneous adipose tissue microRNA expression in response to exercise training in obese African women. Pheiffer C,et al. Scientific Reports, 2022

Re-expression of miR-200s in claudin-low mammary tumor cells alters cell shape and reduces proliferation and invasion potentially through modulating other miRNAs and SUZ12 regulated genes. Simpson K, et al. Cancer Cell International, 2021

Extracellular vesicles derived from myocardial infarction plasma inhibit BMSCs apoptosis and enhance cardiac function via AKT signaling pathway. Jin P, et al. International Immunopharmacology, 2021

Two novel microRNAs and their association with absolute blood pressure parameters in an urban South African community. Matshazi D M, et al. Molecular Biology Reports, 2021

Complexity of the microRNA transcriptome of cow milk and milk-derived extracellular vesicles isolated via differential ultracentrifugation. Benmoussa A, et al. Journal of Dairy Science, 2019

Neuronal microRNA regulation in Experimental Autoimmune Encephalomyelitis. Juzwik C A, et al. Scientific reports, 2018

Defining the rhesus macaque placental miRNAome: Conservation of expression of placental miRNA clusters between the macaque and human. Schmidt J K, et al. Placenta, 2018

MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source. Dias S,et al. BioMed Research International, 2017

Next-generation sequencing of the basal cell carcinoma miRNome and a description of novel microRNA candidates under neoadjuvant vis******* therapy: an integrative molecular and surgical case study.Sand M, et al.Ann Oncol,2016

Integrated analysis of dysregulated ncRNA and mRNA expression profiles in humans exposed to carbon nanotubes. Shvedova A A, et al. PloS one, 2016

Survival and Diversity of Human Homologous Dietary MicroRNAs in Conventionally Cooked Top Sirloin and Dried Bovine Tissue Extracts. Dever J T, et al. PloS one, 2015

RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Hashim A,et al. Oncotarget, 2014

Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks. Melanie Boerries, et al. Kidney International, 2013

More Publications>>

 

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