Gene expression profiling is a technology that utilizes microarrays to analyze genome-wide profiling of gene expression. Arraystar's gene expression profiling service is based on two of the most powerful platforms in this field, including Roche-NimbleGen and Agilent. The availability of the array services from both platforms offers our customers variety of choices in array content, density and analysis tools. The gene expression arrays that are most often selected by our customers include:

Microarray	Species	Array Format	Probe length	Detected genes	Probes /target gene
Whole Human Genome	Human	4 * 44K	60-mer	27,958	1
Whole Mouse Genome	Mouse	4 * 44K	60-mer	39,430	1
Whole Rat Genome	Rat	4 * 44K	60-mer	26,930	1

 

 

 

Microarray	Species	Array Format	Probe length	Detected genes	Probes /target gene
Homo Sapiens 12 * 135K Array	Human	12 * 135K	60-mer	45,033	3
Mus Musculus 12 * 135K v2 Array	Mouse	12 * 135K	60-mer	44,170	3
Rattus Norvegicus 12 * 135K Array	Rat	12 * 135K	60-mer	26,419	5

 

 

Please refer to Sample Submission for details in how to get your project started.

•   RNA isolation (Optional)

•   RNA QC

•   cDNA synthesis

•   Target preparation by labeling with Cy3

•   Array hybridization, washing, and scanning

•   Data extraction, analysis and summarization

 

Arraystar's bioinformatics team has extensive experience in analyzing gene expression array data. We provide two levels of analysis: standard and advanced.

•   Raw Data Files

•   Summary Report

•   Box Plot, Scatter Plot and Hierarchical Clustering

•   Differentially Expressed Gene Screening

•   GO Analysis (Fig.1) and Pathway Analysis

Fig.1 GO Analysis (Standard Data Analysis).  Significant GO terms of differentially expressed gene. A bar plot is generated from the top 10 GO terms containing the largest fold enrichment of differentially expressed genes. The plot gives a more intuitive view of the significant GO terms.